CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009645
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Growth factor receptor-bound protein 2 
Protein Synonyms/Alias
 Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2 
Gene Name
 Grb2 
Gene Synonyms/Alias
 Ash 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
6**MEAIAKYDFKATAacetylation[1]
109GNDVQHFKVLRDGAGubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway (By similarity). 
Sequence Annotation
 DOMAIN 1 58 SH3 1.
 DOMAIN 60 152 SH2.
 DOMAIN 156 215 SH3 2.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 6 6 N6-acetyllysine (By similarity).
 MOD_RES 50 50 N6-acetyllysine (By similarity).
 MOD_RES 109 109 N6-acetyllysine (By similarity).
 MOD_RES 211 211 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Endosome; Golgi apparatus; Nucleus; Phosphoprotein; Reference proteome; Repeat; SH2 domain; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 217 AA 
Protein Sequence
MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK NYIEMKPHPW 60
FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL 120
WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQVPQQPTY VQALFDFDPQ EDGELGFRRG 180
DFIHVMDNSD PNWWKGACHG QTGMFPRNYV TPVNRNV 217 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005768; C:endosome; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0070436; C:Grb2-EGFR complex; IEA:Compara.
 GO:0008180; C:signalosome; ISS:UniProtKB.
 GO:0012506; C:vesicle membrane; IEA:Compara.
 GO:0005154; F:epidermal growth factor receptor binding; ISS:UniProtKB.
 GO:0017124; F:SH3 domain binding; ISS:UniProtKB.
 GO:0007568; P:aging; IPI:RGD.
 GO:0048646; P:anatomical structure formation involved in morphogenesis; IEA:Compara.
 GO:0060670; P:branching involved in labyrinthine layer morphogenesis; IEA:Compara.
 GO:0030154; P:cell differentiation; IEA:Compara.
 GO:0071479; P:cellular response to ionizing radiation; IEA:Compara.
 GO:0008286; P:insulin receptor signaling pathway; IEA:Compara.
 GO:0030838; P:positive regulation of actin filament polymerization; IEA:Compara.
 GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IEA:Compara.
 GO:0051291; P:protein heterooligomerization; IDA:RGD.
 GO:0007265; P:Ras protein signal transduction; TAS:RGD.
 GO:0031623; P:receptor internalization; IEA:Compara.
 GO:0043408; P:regulation of MAPK cascade; IEA:Compara.
 GO:0042770; P:signal transduction in response to DNA damage; IEA:Compara. 
Interpro
 IPR000108; p67phox.
 IPR000980; SH2.
 IPR001452; SH3_domain. 
Pfam
 PF00017; SH2
 PF00018; SH3_1 
SMART
 SM00252; SH2
 SM00326; SH3 
PROSITE
 PS50001; SH2
 PS50002; SH3 
PRINTS
 PR00499; P67PHOX.
 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.