UniProt Accession

 SAE1_MOUSE; Q9R1T2; Q3TRG9; Q3TWJ0; Q9CSW9 

Genbank Protein ID

 AB024303; AK010313; AK011783; AK087556; AK090012; AK154139; AK159672; AK162789; BC068164 

Genbank Nucleotide ID

 BAA82876.1; BAB26845.1; BAB27838.1; BAC39925.1; BAC41044.1; BAE32401.1; BAE35276.1; BAE37060.1; AAH68164.1 

Protein Name

 SUMO-activating enzyme subunit 1 

Protein Synonyms/Alias

 Ubiquitin-like 1-activating enzyme E1A; SUMO-activating enzyme subunit 1, N-terminally processed 

Gene Name

 Sae1 

Gene Synonyms/Alias

 Aos1; Sua1; Ubl1a1; Uble1a 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
36	LWGLEAQKRLRASRV	acetylation	[1]
36	LWGLEAQKRLRASRV	ubiquitination	[2]
145	CSRDVIIKVDQICHR	acetylation	[1]
187	EEKTKVAKVSQGVED	ubiquitination	[2]
199	VEDGPEAKRAKLDSS	ubiquitination	[2]
202	GPEAKRAKLDSSETT	acetylation	[3]
212	SSETTMVKKKVLFCP	ubiquitination	[2]
232	EVDWSGEKAKAALKR	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity). 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylvaline; in SUMO-activating enzyme  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Ligase; Nucleus; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 350 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008022; F:protein C-terminus binding; ISS:UniProtKB.
 GO:0019948; F:SUMO activating enzyme activity; IEA:Compara.
 GO:0016925; P:protein sumoylation; ISS:UniProtKB. 

Interpro

 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR000011; UBQ/SUMO-activ_enz_E1-like. 

Pfam

 PF00899; ThiF 

SMART

  

PROSITE

  

PRINTS

 PR01849; UBIQUITINACT.