CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009375
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Syntaxin-binding protein 1 
Protein Synonyms/Alias
 MUNC18-1; N-Sec1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A; p67 
Gene Name
 STXBP1 
Gene Synonyms/Alias
 UNC18A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MAPIGLKAVVGEKIubiquitination[1]
13LKAVVGEKIMHDVIKubiquitination[1, 2]
208LAQLIQDKLDAYKADubiquitination[3]
314LKDFSSSKRMNTGEKubiquitination[1]
321KRMNTGEKTTMRDLSmethylation[4]
321KRMNTGEKTTMRDLSubiquitination[1]
339KKMPQYQKELSKYSTubiquitination[1]
364HYQGTVDKLCRVEQDubiquitination[1]
587DTLKKLNKTDEEISSubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 
Functional Description
 May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. 
Sequence Annotation
 MOD_RES 107 107 Phosphothreonine (By similarity).
 MOD_RES 142 142 Phosphoserine (By similarity).
 MOD_RES 145 145 Phosphotyrosine (By similarity).
 MOD_RES 146 146 Phosphoserine (By similarity).
 MOD_RES 345 345 Phosphoserine (By similarity).
 MOD_RES 346 346 Phosphothreonine (By similarity).
 MOD_RES 473 473 Phosphotyrosine (By similarity).
 MOD_RES 507 507 Phosphoserine (By similarity).
 MOD_RES 509 509 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; Epilepsy; Membrane; Mental retardation; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 594 AA 
Protein Sequence
MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI MTEGITIVED 60
INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR AAHVFFTDSC PDALFNELVK 120
SRAAKVIKTL TEINIAFLPY ESQVYSLDSA DSFQSFYSPH KAQMKNPILE RLAEQIATLC 180
ATLKEYPAVR YRGEYKDNAL LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS 240
SPVLHELTFQ AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS 300
QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL AEDCMKHYQG 360
TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS TYDKIRIILL YIFLKNGITE 420
ENLNKLIQHA QIPPEDSEII TNMAHLGVPI VTDSTLRRRS KPERKERISE QTYQLSRWTP 480
IIKDIMEDTI EDKLDTKHYP YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF 540
ILGGVSLNEM RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS 594 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0031091; C:platelet alpha granule; IDA:UniProtKB.
 GO:0043234; C:protein complex; ISS:UniProtKB.
 GO:0042802; F:identical protein binding; ISS:UniProtKB.
 GO:0017075; F:syntaxin-1 binding; ISS:UniProtKB.
 GO:0007412; P:axon target recognition; ISS:UniProtKB.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0014047; P:glutamate secretion; TAS:Reactome.
 GO:0060292; P:long term synaptic depression; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0032229; P:negative regulation of synaptic transmission, GABAergic; ISS:UniProtKB.
 GO:0007274; P:neuromuscular synaptic transmission; IEA:Compara.
 GO:0007269; P:neurotransmitter secretion; TAS:Reactome.
 GO:0070527; P:platelet aggregation; IMP:UniProtKB.
 GO:0002576; P:platelet degranulation; IMP:UniProtKB.
 GO:0045956; P:positive regulation of calcium ion-dependent exocytosis; IEA:Compara.
 GO:0050821; P:protein stabilization; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0010807; P:regulation of synaptic vesicle priming; ISS:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0016188; P:synaptic vesicle maturation; ISS:UniProtKB.
 GO:0006904; P:vesicle docking involved in exocytosis; IEA:Compara. 
Interpro
 IPR027482; Sec-1-like_dom2.
 IPR001619; Sec1-like. 
Pfam
 PF00995; Sec1 
SMART
  
PROSITE
  
PRINTS