UniProt Accession

 UCHL1_HUMAN; P09936; Q4W5K6; Q71UM0 

Genbank Protein ID

 AC095043; CH471069; BC000332; BC005117; BC006305; X17377; X04741; AH007277 

Genbank Nucleotide ID

 AAY40923.1; EAW92983.1; AAH00332.1; AAH05117.1; AAH06305.1; CAA35249.1; CAA28443.1; AAD09172.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase isozyme L1 

Protein Synonyms/Alias

 UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1 

Gene Name

 UCHL1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MQLKPMEINPE	ubiquitination	[1, 2, 3, 4]
15	INPEMLNKVLSRLGV	ubiquitination	[2]
65	QHENFRKKQIEELKG	ubiquitination	[1, 3, 4]
71	KKQIEELKGQEVSPK	ubiquitination	[1, 3, 4]
78	KGQEVSPKVYFMKQT	ubiquitination	[2, 4]
83	SPKVYFMKQTIGNSC	ubiquitination	[2]
115	FEDGSVLKQFLSETE	ubiquitination	[2, 3, 4, 5]
123	QFLSETEKMSPEDRA	ubiquitination	[4]
135	DRAKCFEKNEAIQAA	ubiquitination	[3, 4]
157	GQCRVDDKVNFHFIL	ubiquitination	[1, 3, 4, 6]
195	SSEDTLLKDAAKVCR	acetylation	[7]
195	SSEDTLLKDAAKVCR	ubiquitination	[4]

Reference

 [1] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. 

Sequence Annotation

 REGION 5 10 Interaction with ubiquitin.
 REGION 211 216 Interaction with ubiquitin.
 ACT_SITE 90 90 Nucleophile.
 ACT_SITE 161 161 Proton donor.
 LIPID 220 220 S-farnesyl cysteine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Hydrolase; Ligase; Lipoprotein; Membrane; Neurodegeneration; Oxidation; Parkinson disease; Parkinsonism; Polymorphism; Prenylation; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IEA:Compara.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0004197; F:cysteine-type endopeptidase activity; IDA:UniProtKB.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008242; F:omega peptidase activity; IDA:UniProtKB.
 GO:0043130; F:ubiquitin binding; IDA:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; TAS:UniProtKB.
 GO:0007628; P:adult walking behavior; IEA:Compara.
 GO:0007412; P:axon target recognition; IEA:Compara.
 GO:0019896; P:axon transport of mitochondrion; IEA:Compara.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0042755; P:eating behavior; IEA:Compara.
 GO:0048747; P:muscle fiber development; IEA:Compara.
 GO:0043407; P:negative regulation of MAP kinase activity; IDA:BHF-UCL.
 GO:0050905; P:neuromuscular process; IEA:Compara.
 GO:0016579; P:protein deubiquitination; IDA:UniProtKB.
 GO:0006950; P:response to stress; IEA:Compara.
 GO:0019233; P:sensory perception of pain; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:Compara. 

Interpro

 IPR001578; Peptidase_C12. 

Pfam

 PF01088; Peptidase_C12 

SMART

  

PROSITE

 PS00140; UCH_1 

PRINTS

 PR00707; UBCTHYDRLASE.