CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001098
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-like protein KIF21B 
Protein Synonyms/Alias
  
Gene Name
 KIF21B 
Gene Synonyms/Alias
 KIAA0449 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
205EELIQCLKQGALSRTubiquitination[1]
677KLILLQNKIRDTQLEacetylation[2]
702MECYTEEKANKIKADacetylation[3]
1051KGLQVAQKEAQIRLLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Microtubule-binding motor protein probably involved in neuronal dendritic transport. In vitro, has a plus-end directed motor activity (By similarity). 
Sequence Annotation
 DOMAIN 5 298 Kinesin-motor.
 REPEAT 1306 1343 WD 1.
 REPEAT 1346 1384 WD 2.
 REPEAT 1410 1448 WD 3.
 REPEAT 1451 1493 WD 4.
 REPEAT 1502 1539 WD 5.
 REPEAT 1543 1582 WD 6.
 REPEAT 1585 1622 WD 7.
 NP_BIND 87 94 ATP (By similarity).
 MOD_RES 579 579 Phosphoserine (By similarity).
 MOD_RES 582 582 Phosphothreonine (By similarity).
 MOD_RES 1167 1167 Phosphoserine.
 MOD_RES 1237 1237 Phosphothreonine.
 MOD_RES 1241 1241 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Motor protein; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1637 AA 
Protein Sequence
MAGQGDCCVK VAVRIRPQLS KEKIEGCHIC TSVTPGEPQV LLGKDKAFTY DFVFDLDTWQ 60
EQIYSTCVSK LIEGCFEGYN ATVLAYGQTG AGKTYTMGTG FDMATSEEEQ GIIPRAIAHL 120
FGGIAERKRR AQEQGVAGPE FKVSAQFLEL YNEEILDLFD STRDPDTRHR RSNIKIHEDA 180
NGGIYTTGVT SRLIHSQEEL IQCLKQGALS RTTASTQMNV QSSRSHAIFT IHLCQMRMCT 240
QPDLVNEAVT GLPDGTPPSS EYETLTAKFH FVDLAGSERL KRTGATGERA KEGISINCGL 300
LALGNVISAL GDQSKKVVHV PYRDSKLTRL LQDSLGGNSQ TIMIACVSPS DRDFMETLNT 360
LKYANRARNI KNKVVVNQDK TSQQISALRA EIARLQMELM EYKAGKRVIG EDGAEGYSDL 420
FRENAMLQKE NGALRLRVKA MQEAIDAINN RVTQLMSQEA NLLLAKAGDG NEAIGALIQN 480
YIREIEELRT KLLESEAMNE SLRRSLSRAS ARSPYSLGAS PAAPAFGGSP ASSMEDASEV 540
IRRAKQDLER LKKKEVRQRR KSPEKEAFKK RAKLQQENSE ETDENEAEEE EEERDESGCE 600
EEEGREDEDE DSGSEESLVD SDSDPEEKEV NFQADLADLT CEIEIKQKLI DELENSQRRL 660
QTLKHQYEEK LILLQNKIRD TQLERDRVLQ NLSTMECYTE EKANKIKADY EKRLREMNRD 720
LQKLQAAQKE HARLLKNQSR YERELKKLQA EVAEMKKAKV ALMKQMREEQ QRRRLVETKR 780
NREIAQLKKE QRRQEFQIRA LESQKRQQEM VLRRKTQEVS ALRRLAKPMS ERVAGRAGLK 840
PPMLDSGAEV SASTTSSEAE SGARSVSSIV RQWNRKINHF LGDHPAPTVN GTRPARKKFQ 900
KKGASQSFSK AARLKWQSLE RRIIDIVMQR MTIVNLEADM ERLIKKREEL FLLQEALRRK 960
RERLQAESPE EEKGLQELAE EIEVLAANID YINDGITDCQ ATIVQLEETK EELDSTDTSV 1020
VISSCSLAEA RLLLDNFLKA SIDKGLQVAQ KEAQIRLLEG RLRQTDMAGS SQNHLLLDAL 1080
REKAEAHPEL QALIYNVQQE NGYASTDEEI SEFSEGSFSQ SFTMKGSTSH DDFKFKSEPK 1140
LSAQMKAVSA ECLGPPLDIS TKNITKSLAS LVEIKEDGVG FSVRDPYYRD RVSRTVSLPT 1200
RGSTFPRQSR ATETSPLTRR KSYDRGQPIR STDVGFTPPS SPPTRPRNDR NVFSRLTSNQ 1260
SQGSALDKSD DSDSSLSEVL RGIISPVGGA KGARTAPLQC VSMAEGHTKP ILCLDATDEL 1320
LFTGSKDRSC KMWNLVTGQE IAALKGHPNN VVSIKYCSHS GLVFSVSTSY IKVWDIRDSA 1380
KCIRTLTSSG QVISGDACAA TSTRAITSAQ GEHQINQIAL SPSGTMLYAA SGNAVRIWEL 1440
SRFQPVGKLT GHIGPVMCLT VTQTASQHDL VVTGSKDHYV KMFELGECVT GTIGPTHNFE 1500
PPHYDGIECL AIQGDILFSG SRDNGIKKWD LDQQELIQQI PNAHKDWVCA LAFIPGRPML 1560
LSACRAGVIK VWNVDNFTPI GEIKGHDSPI NAICTNAKHI FTASSDCRVK LWNYVPGLTP 1620
CLPRRVLAIK GRATTLP 1637 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005875; C:microtubule associated complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003777; F:microtubule motor activity; IEA:InterPro.
 GO:0007018; P:microtubule-based movement; IEA:InterPro. 
Interpro
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase.
 IPR009053; Prefoldin.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00225; Kinesin
 PF00400; WD40 
SMART
 SM00129; KISc
 SM00320; WD40 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00380; KINESINHEAVY.