UniProt Accession

 ARO8_YEAST; P53090; D6VTV2 

Genbank Protein ID

 Y13624; Z72724; AY692962; BK006941 

Genbank Nucleotide ID

 CAA73946.1; CAA96914.1; AAT92981.1; DAA07913.1 

Protein Name

 Aromatic/aminoadipate aminotransferase 1 

Protein Synonyms/Alias

 2-aminoadipate aminotransferase; 2-aminoadipate transaminase; Alpha-aminoadipate aminotransferase; AadAT; Aromatic amino acid aminotransferase 1; Aromatic amino acid aminotransferase I; Aromatic amino acid-requiring protein 8 

Gene Name

 ARO8 

Gene Synonyms/Alias

 YGL202W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
89	VNKDYADKSANPSND	acetylation	[1]
89	VNKDYADKSANPSND	ubiquitination	[2]
123	NFIRDHTKIIHDLKY	acetylation	[1]
129	TKIIHDLKYKDWDVL	acetylation	[1]
371	LRHEYTLKRDCAIDA	acetylation	[1]
415	VHPEFKTKYNSDPYQ	acetylation	[1]
430	LEQSLYHKVVERGVL	ubiquitination	[2]
445	VVPGSWFKSEGETEP	ubiquitination	[2]
480	YAAVSPEKLTEGLKR	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 General aromatic amino acid transaminase involved in several otherwise unrelated metabolic pathways. Responsible for phenylalanine and tyrosine biosynthesis. Active with glutamate, phenylalanine, tyrosine and tryptophan as amino donors and with phenylpyruvate, hydroxyphenylpyruvate, 2-oxoglutarate and pyruvate as amino acceptors. Also active with methionine, alpha- aminoadipate and leucine as amino donors when phenylpyruvate is the amino acceptor and in the reverse reactions with the corresponding oxo acids and phenylalanine as amino donor. Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using aromatic amino acids (tyrosine, phenylalanine and tryptophan) as the amino donors. Catalyzes the formation of alpha- aminoadipate from alpha-ketoadipate in the lysine biosyntheic pathway. 

Sequence Annotation

 MOD_RES 100 100 Phosphoserine.  

Keyword

 Aminotransferase; Complete proteome; Cytoplasm; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 500 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0047536; F:2-aminoadipate transaminase activity; IDA:SGD.
 GO:0008793; F:aromatic-amino-acid:2-oxoglutarate aminotransferase activity; IDA:SGD.
 GO:0080130; F:L-phenylalanine:2-oxoglutarate aminotransferase activity; IEA:EC.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0009072; P:aromatic amino acid family metabolic process; IGI:SGD.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; IEA:UniProtKB-UniPathway.
 GO:0009094; P:L-phenylalanine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0019878; P:lysine biosynthetic process via aminoadipic acid; IEA:UniProtKB-UniPathway.
 GO:0006571; P:tyrosine biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR004839; Aminotransferase_I/II.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 

Pfam

 PF00155; Aminotran_1_2 

SMART

  

PROSITE

  

PRINTS