CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007149
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydroxy-acid dehydratase, mitochondrial 
Protein Synonyms/Alias
 DAD; 2,3-dihydroxy acid hydrolyase 
Gene Name
 ILV3 
Gene Synonyms/Alias
 YJR016C; J1450 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
25CVAKKLNKYSYIITEacetylation[1]
34SYIITEPKGQGASQAacetylation[1]
49MLYATGFKKEDFKKPacetylation[1]
109DGISMGTKGMRYSLQacetylation[1]
266DNIGEYIKKTMELGIacetylation[1]
334TPLIGDFKPSGKYVMacetylation[1]
393PEGQEIIKPLSHPIKacetylation[1]
424AVGKITGKEGTYFKGacetylation[1]
430GKEGTYFKGRARVFEacetylation[1]
477PGMPEMLKPSSALMGacetylation[1]
489LMGYGLGKDVALLTDacetylation[1]
546IDLLVSDKEMAQRKQacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
  
Sequence Annotation
 METAL 143 143 Iron-sulfur (4Fe-4S) (Potential).
 METAL 221 221 Iron-sulfur (4Fe-4S) (Potential).  
Keyword
 4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Iron; Iron-sulfur; Lyase; Metal-binding; Mitochondrion; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MGLLTKVATS RQFSTTRCVA KKLNKYSYII TEPKGQGASQ AMLYATGFKK EDFKKPQVGV 60
GSCWWSGNPC NMHLLDLNNR CSQSIEKAGL KAMQFNTIGV SDGISMGTKG MRYSLQSREI 120
IADSFETIMM AQHYDANIAI PSCDKNMPGV MMAMGRHNRP SIMVYGGTIL PGHPTCGSSK 180
ISKNIDIVSA FQSYGEYISK QFTEEEREDV VEHACPGPGS CGGMYTANTM ASAAEVLGLT 240
IPNSSSFPAV SKEKLAECDN IGEYIKKTME LGILPRDILT KEAFENAITY VVATGGSTNA 300
VLHLVAVAHS AGVKLSPDDF QRISDTTPLI GDFKPSGKYV MADLINVGGT QSVIKYLYEN 360
NMLHGNTMTV TGDTLAERAK KAPSLPEGQE IIKPLSHPIK ANGHLQILYG SLAPGGAVGK 420
ITGKEGTYFK GRARVFEEEG AFIEALERGE IKKGEKTVVV IRYEGPRGAP GMPEMLKPSS 480
ALMGYGLGKD VALLTDGRFS GGSHGFLIGH IVPEAAEGGP IGLVRDGDEI IIDADNNKID 540
LLVSDKEMAQ RKQSWVAPPP RYTRGTLSKY AKLVSNASNG CVLDA 585 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0004160; F:dihydroxy-acid dehydratase activity; IMP:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009082; P:branched-chain amino acid biosynthetic process; IMP:SGD.
 GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR015928; Aconitase/3IPM_dehydase_swvl.
 IPR004404; DihydroxyA_deHydtase.
 IPR000581; DiOHA_6PGluconate_deHydtase.
 IPR020558; DiOHA_6PGluconate_deHydtase_CS. 
Pfam
 PF00920; ILVD_EDD 
SMART
  
PROSITE
 PS00886; ILVD_EDD_1
 PS00887; ILVD_EDD_2 
PRINTS