CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043109
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cholinergic receptor, nicotinic, alpha 5, isoform CRA_b 
Protein Synonyms/Alias
 Neuronal acetylcholine receptor subunit alpha-5 
Gene Name
 CHRNA5 
Gene Synonyms/Alias
 hCG_95858 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45SEPSSIAKHEDSLLKubiquitination[1]
52KHEDSLLKDLFQDYEubiquitination[1, 2, 3, 4]
71PVEHLNDKIKIKFGLubiquitination[1]
107KQEWIDVKLRWNPDDubiquitination[1, 2, 3]
119PDDYGGIKVIRVPSDubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Cell junction; Cell membrane; Complete proteome; Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Postsynaptic cell membrane; Receptor; Reference proteome; Synapse; Transmembrane; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 160 AA 
Protein Sequence
MAARGSGPRA LRLLLLVQLV AGRCGLAGAA GGAQRGLSEP SSIAKHEDSL LKDLFQDYER 60
WVRPVEHLND KIKIKFGLAI SQLVDVDEKN QLMTTNVWLK QEWIDVKLRW NPDDYGGIKV 120
IRVPSDSVWT PDIVLFDNAD GRFEGTSTKT VIRLLKIGNS 160 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0004889; F:acetylcholine-activated cation-selective channel activity; IEA:InterPro. 
Interpro
 IPR006202; Neur_chan_lig-bd.
 IPR006201; Neur_channel.
 IPR002394; Nicotinic_acetylcholine_rcpt. 
Pfam
 PF02931; Neur_chan_LBD 
SMART
  
PROSITE
  
PRINTS
 PR00254; NICOTINICR.