UniProt Accession

 PRS8_YEAST; Q01939; D6VU92 

Genbank Protein ID

 X66400; L01626; Z72570; AY693135; BK006941 

Genbank Nucleotide ID

 CAA47023.1; AAA35138.1; CAA96750.1; AAT93154.1; DAA08053.1 

Protein Name

 26S protease regulatory subunit 8 homolog 

Protein Synonyms/Alias

 Protein CIM3; Protein SUG1; Tat-binding protein TBY1 

Gene Name

 RPT6 

Gene Synonyms/Alias

 CIM3; CRL3; SUG1; TBPY; TBY1; YGL048C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
93	VKVQPEGKYIVDVAK	ubiquitination	[1]
100	KYIVDVAKDINVKDL	acetylation	[2]
195	YGPPGTGKTLLARAV	ubiquitination	[1]
209	VAHHTDCKFIRVSGA	acetylation	[2]
221	SGAELVQKYIGEGSR	acetylation	[2]
221	SGAELVQKYIGEGSR	ubiquitination	[1]
286	LDGFETSKNIKIIMA	ubiquitination	[1]
349	NLRKVAEKMNGCSGA	ubiquitination	[1]
359	GCSGADVKGVCTEAG	ubiquitination	[1]
392	AVGKVMNKNQETAIS	ubiquitination	[1]
402	ETAISVAKLFK****	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). 

Sequence Annotation

 NP_BIND 189 196 ATP.
 MOD_RES 2 2 N-acetylthreonine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Nucleus; Proteasome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 405 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:SGD.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; ISS:SGD.
 GO:0019904; F:protein domain specific binding; IDA:SGD.
 GO:0006338; P:chromatin remodeling; IMP:SGD.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IMP:SGD.
 GO:0006289; P:nucleotide-excision repair; IGI:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IMP:SGD.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IMP:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IPI:SGD.
 GO:0070682; P:proteasome regulatory particle assembly; IMP:SGD. 

Interpro

 IPR005937; 26S_Psome_P45.
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00004; AAA 

SMART

 SM00382; AAA 

PROSITE

 PS00674; AAA 

PRINTS