UniProt Accession

 DCTN2_HUMAN; Q13561; B2RBK5; Q86YN2; Q9BW17 

Genbank Protein ID

 U50733; AK314705; AC022366; AC022506; BC000718; BC009468; BC014083; AY189155 

Genbank Nucleotide ID

 AAC50423.1; BAG37252.1; AAH00718.1; AAH09468.1; AAH14083.1; AAO34395.1 

Protein Name

 Dynactin subunit 2 

Protein Synonyms/Alias

 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; p50 dynamitin 

Gene Name

 DCTN2 

Gene Synonyms/Alias

 DCTN50 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MADPKYADLPGI	ubiquitination	[1]
140	LTPVLLAKQLAALKQ	ubiquitination	[1, 2]
146	AKQLAALKQQLVASH	ubiquitination	[2]
175	DPDGALAKRLLLQLE	ubiquitination	[3]
297	VNEIAKHKASVEDAD	ubiquitination	[3]
308	EDADTQSKVHQLYET	ubiquitination	[3]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 6 6 Phosphotyrosine.
 MOD_RES 83 83 Phosphoserine.
 MOD_RES 86 86 Phosphotyrosine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Dynein; Membrane; Microtubule; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 401 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005869; C:dynactin complex; IDA:UniProtKB.
 GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0031982; C:vesicle; IDA:UniProtKB.
 GO:0003774; F:motor activity; IEA:UniProtKB-KW.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0008283; P:cell proliferation; IMP:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007067; P:mitosis; IMP:UniProtKB.
 GO:0007052; P:mitotic spindle organization; IEA:Compara. 

Interpro

 IPR006996; Dynamitin_2su. 

Pfam

  

SMART

  

PROSITE

  

PRINTS