CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010112
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 DNA primase 
Protein Synonyms/Alias
  
Gene Name
 dnaG 
Gene Synonyms/Alias
 dnaE; MPN_353; MP483 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
234FSGRALQKTEKIKYKacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis. 
Sequence Annotation
 DOMAIN 266 350 Toprim.
 ZN_FING 38 62 CHC2-type (By similarity).  
Keyword
 Complete proteome; DNA replication; DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase; Primosome; Reference proteome; Transcription; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 620 AA 
Protein Sequence
MTSPTSLDQL KQQIKIAPIV EHYAIKLKKK GKDFVALCPF HADQNPSMTV SVAKNIFKCF 60
SCQVGGDGIA FIQKIDQVDW KTALNKALSI LNLDSQYAVN FYLKEVDPKL KRYWDLHSAL 120
VDYYQTRLKL EPKEQGLTYL TETRKLSPQV IERFQLGLAF TLEDQYFLPS LLNYPWISPA 180
IEKAELCFAT EKFPEALGFF NQQTHYATFK SRIMIPIHDL KGNPVGFSGR ALQKTEKIKY 240
KNSAEHQWFK KSELLFNFHR IDKNTLKLYL VEGYFDVFAL TSAGIGDVVG LMGLALSESH 300
IIAFQQQLKA LETVVLALDN DTAGHDATFK LLQELNAHGI IVEVVDWNQA AYKDWDELFL 360
AEGSDAVKAK THRVLNLVEY LVAYFKTKGF DERITVNKVI DIIAQNQKVT ADTSFSRFLC 420
QKLQQLLQYS DVETLFTQLQ QQKLKVKVNK TTTFTQRAPI YESVVGVVDN SFRNESQPVA 480
ITKEFLVENN WKETKERVFH AEIFAYVLLD KQFLVELKQS DLDELFASLQ TPLFDVALFI 540
DKARLYWAKV QEPDWAVFNS ILGEQQAMFP TTFLAQIKEF FLNKSLSYDP EDYEEDLQFF 600
RQLIVKQKEL LKYFKSMVEH 620 
Gene Ontology
 GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003896; F:DNA primase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR013264; DNA_primase_core_N.
 IPR006295; DNA_primase_DnaG.
 IPR006171; Toprim_domain.
 IPR002694; Znf_CHC2. 
Pfam
 PF01751; Toprim
 PF08275; Toprim_N
 PF01807; zf-CHC2 
SMART
 SM00493; TOPRIM
 SM00400; ZnF_CHCC 
PROSITE
 PS50880; TOPRIM 
PRINTS