UniProt Accession

 MURE_ECOLI; P22188; O07101 

Genbank Protein ID

 X55814; X55034; U00096; AP009048; U67894 

Genbank Nucleotide ID

 CAA39334.1; CAA38862.1; AAC73196.1; BAB96653.1; AAB60789.1 

Protein Name

 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase 

Protein Synonyms/Alias

 Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase 

Gene Name

 murE 

Gene Synonyms/Alias

 b0085; JW0083 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
252	VGRRWLAKLPDAVAV	acetylation	[1]
367	HTPDALEKALQAARL	acetylation	[1]
439	MLDAGHAKVMEGRAE	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. Is also able to use many meso-diaminopimelate analogs as substrates, although much less efficiently, but not L-lysine. 

Sequence Annotation

 NP_BIND 116 122 ATP (Potential).
 REGION 44 46 UDP-MurNAc-L-Ala-D-Glu binding.
 REGION 158 159 UDP-MurNAc-L-Ala-D-Glu binding.
 REGION 414 417 Meso-diaminopimelate binding.
 MOTIF 414 417 Meso-diaminopimelate recognition motif.
 BINDING 27 27 UDP-MurNAc-L-Ala-D-Glu; via carbonyl
 BINDING 29 29 UDP-MurNAc-L-Ala-D-Glu.
 BINDING 157 157 UDP-MurNAc-L-Ala-D-Glu.
 BINDING 185 185 UDP-MurNAc-L-Ala-D-Glu.
 BINDING 191 191 UDP-MurNAc-L-Ala-D-Glu.
 BINDING 193 193 UDP-MurNAc-L-Ala-D-Glu.
 BINDING 390 390 Meso-diaminopimelate.
 BINDING 465 465 Meso-diaminopimelate; via carbonyl
 BINDING 469 469 Meso-diaminopimelate.
 MOD_RES 225 225 N6-carboxylysine.  

Keyword

 3D-structure; ATP-binding; Cell cycle; Cell division; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Peptidoglycan synthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 495 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0047482; F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase activity; IDA:EcoliWiki.
 GO:0008765; F:UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity; IEA:HAMAP.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. 

Interpro

 IPR004101; Mur_ligase_C.
 IPR013221; Mur_ligase_cen.
 IPR000713; Mur_ligase_N.
 IPR005761; UDP-N-AcMur-Glu-dNH2Pim_ligase. 

Pfam

 PF01225; Mur_ligase
 PF02875; Mur_ligase_C
 PF08245; Mur_ligase_M 

SMART

  

PROSITE

  

PRINTS