CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012835
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WASH complex subunit 7 
Protein Synonyms/Alias
  
Gene Name
 KIAA1033 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
29IHAEVQLKNYGKFLEubiquitination[1, 2]
33VQLKNYGKFLEEYTSubiquitination[2]
233SVHHNPSKFGIQEEKubiquitination[1, 2, 3]
240KFGIQEEKLKPFEKFubiquitination[2]
302IFANVEAKLGEPSEIubiquitination[1]
373QKIPAAAKLLDRKSLubiquitination[2]
394RDTFLQQKAQSLTKDubiquitination[1]
422KMESILSKEQRMDKFubiquitination[1]
553TLNGPSTKQRRLIVSubiquitination[1, 4]
573GTQMKTFKDEELFPLubiquitination[1, 2]
585FPLQVVMKKLDLISEubiquitination[1]
586PLQVVMKKLDLISELubiquitination[1]
813IERTSNNKHLNTINIubiquitination[1, 2, 3]
877IRFFREIKDQNDHKYubiquitination[2]
968GLAEETLKAARHLDSubiquitination[1]
1082SVREKYLKEIRAVAKubiquitination[2]
1089KEIRAVAKQQNVQSAubiquitination[1, 2, 3, 5]
1111QTMNLTQKRLDVYLQubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 7 7 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1173 AA 
Protein Sequence
MAVETLSPDW EFDRVDDGSQ KIHAEVQLKN YGKFLEEYTS QLRRIEDALD DSIGDVWDFN 60
LDPIALKLLP YEQSSLLELI KTENKVLNKV ITVYAALCCE IKKLKYEAET KFYNGLLFYG 120
EGATDASMVE GDCQIQMGRF ISFLQELSCF VTRCYEVVMN VVHQLAALYI SNKIAPKIIE 180
TTGVHFQTMY EHLGELLTVL LTLDEIIDNH ITLKDHWTMY KRLLKSVHHN PSKFGIQEEK 240
LKPFEKFLLK LEGQLLDGMI FQACIEQQFD SLNGGVSVSK NSTFAEEFAH SIRSIFANVE 300
AKLGEPSEID QRDKYVGICG LFVLHFQIFR TIDKKFYKSL LDICKKVPAI TLTANIIWFP 360
DNFLIQKIPA AAKLLDRKSL QAIKIHRDTF LQQKAQSLTK DVQSYYVFVS SWMMKMESIL 420
SKEQRMDKFA EDLTNRCNVF IQGFLYAYSI STIIKTTMNL YMSMQKPMTK TSVKALCRLV 480
ELLKAIEHMF YRRSMVVADS VSHITQHLQH QALHSISVAK KRVISDKKYS EQRLDVLSAL 540
VLAENTLNGP STKQRRLIVS LALSVGTQMK TFKDEELFPL QVVMKKLDLI SELRERVQTQ 600
CDCCFLYWHR AVFPIYLDDV YENAVDAARL HYMFSALRDC VPAMMHARHL ESYEILLDCY 660
DKEIMEILNE HLLDKLCKEI EKDLRLSVHT HLKLDDRNPF KVGMKDLALF FSLNPIRFFN 720
RFIDIRAYVT HYLDKTFYNL TTVALHDWAT YSEMRNLATQ RYGLVMTEAH LPSQTLEQGL 780
DVLEIMRNIH IFVSRYLYNL NNQIFIERTS NNKHLNTINI RHIANSIRTH GTGIMNTTVN 840
FTYQFLKKKF YIFSQFMYDE HIKSRLIKDI RFFREIKDQN DHKYPFDRAE KFNRGIRKLG 900
VTPEGQSYLD QFRQLISQIG NAMGYVRMIR SGGLHCSSNA IRFVPDLEDI VNFEELVKEE 960
GLAEETLKAA RHLDSVLSDH TRNSAEGTEY FKMLVDVFAP EFRRPKNIHL RNFYIIVPPL 1020
TLNFVEHSIS CKEKLNKKNK IGAAFTDDGF AMGVAYILKL LDQYREFDSL HWFQSVREKY 1080
LKEIRAVAKQ QNVQSASQDE KLLQTMNLTQ KRLDVYLQEF ELLYFSLSSA RIFFRADKTA 1140
AEENQEKKEK EEETKTSNGD LSDSTVSADP VVK 1173 
Gene Ontology
 GO:0005768; C:endosome; IDA:MGI.
 GO:0071203; C:WASH complex; IDA:UniProtKB.
 GO:0016197; P:endosomal transport; ISS:UniProtKB. 
Interpro
 IPR027307; WASH7. 
Pfam
  
SMART
  
PROSITE
  
PRINTS