CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009166
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-40S ribosomal protein S27a-1 
Protein Synonyms/Alias
 Ubiquitin; 40S ribosomal protein S27a-1 
Gene Name
 RPS27AA 
Gene Synonyms/Alias
 UBQ16; At1g23410; F26F24.28; F28C11.5 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
11FVKTLTGKTITLEVEubiquitination[1]
29TIDNVKAKIQDKEGIubiquitination[1]
33VKAKIQDKEGIPPDQubiquitination[1]
48QRLIFAGKQLEDGRTubiquitination[1]
63LADYNIQKESTLHLVubiquitination[1]
Reference
 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265
Functional Description
 Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA- damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). 
Sequence Annotation
 DOMAIN 1 76 Ubiquitin-like.
 ZN_FING 121 144 C4-type.
 CROSSLNK 11 11 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 29 29 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 33 33 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 48 48 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 63 63 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 76 76 Glycyl lysine isopeptide (Gly-Lys)  
Keyword
 Complete proteome; Cytoplasm; Isopeptide bond; Metal-binding; Nucleus; Reference proteome; Ribonucleoprotein; Ribosomal protein; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 156 AA 
Protein Sequence
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLADYN 60
IQKESTLHLV LRLRGGAKKR KKKTYTKPKK IKHTHKKVKL AVLQFYKVDG SGKVQRLKKE 120
CPSVSCGPGT FMASHFDRHY CGKCGTTYVF KKADEE 156 
Gene Ontology
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:TAIR.
 GO:0005794; C:Golgi apparatus; IDA:TAIR.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:TAIR.
 GO:0009506; C:plasmodesma; IDA:TAIR.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0006412; P:translation; IEA:InterPro. 
Interpro
 IPR002906; Ribosomal_S27a.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019956; Ubiquitin_subgr.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF01599; Ribosomal_S27
 PF00240; ubiquitin 
SMART
 SM00213; UBQ 
PROSITE
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 
PRINTS
 PR00348; UBIQUITIN.