CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008196
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA-binding protein 25 
Protein Synonyms/Alias
 Arg/Glu/Asp-rich protein of 120 kDa; RED120; Protein S164; RNA-binding motif protein 25; RNA-binding region-containing protein 7 
Gene Name
 RBM25 
Gene Synonyms/Alias
 RNPC7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
477KKTREYEKEAEREEEubiquitination[1]
578RRRQPQIKQEPESEEsumoylation[2]
685PGQPNSVKRKKLPVDubiquitination[3]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. 
Sequence Annotation
 DOMAIN 87 164 RRM.
 DOMAIN 750 843 PWI.
 REGION 285 644 Necessary for nuclear speckle
 MOD_RES 583 583 Phosphoserine.
 MOD_RES 677 677 Phosphoserine.
 MOD_RES 683 683 Phosphoserine.
 MOD_RES 703 703 Phosphoserine.
 CROSSLNK 477 477 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 843 AA 
Protein Sequence
MSFPPHLNRP PMGIPALPPG IPPPQFPGFP PPVPPGTPMI PVPMSIMAPA PTVLVPTVSM 60
VGKHLGARKD HPGLKAKEND ENCGPTTTVF VGNISEKASD MLIRQLLAKC GLVLSWKRVQ 120
GASGKLQAFG FCEYKEPEST LRALRLLHDL QIGEKKLLVK VDAKTKAQLD EWKAKKKASN 180
GNARPETVTN DDEEALDEET KRRDQMIKGA IEVLIREYSS ELNAPSQESD SHPRKKKKEK 240
KEDIFRRFPV APLIPYPLIT KEDINAIEME EDKRDLISRE ISKFRDTHKK LEEEKGKKEK 300
ERQEIEKERR ERERERERER ERRERERERE REREREKEKE RERERERDRD RDRTKERDRD 360
RDRERDRDRD RERSSDRNKD RSRSREKSRD RERERERERE RERERERERE RERERERERE 420
REREREKDKK RDREEDEEDA YERRKLERKL REKEAAYQER LKNWEIRERK KTREYEKEAE 480
REEERRREMA KEAKRLKEFL EDYDDDRDDP KYYRGSALQK RLRDREKEME ADERDRKREK 540
EELEEIRQRL LAEGHPDPDA ELQRMEQEAE RRRQPQIKQE PESEEEEEEK QEKEEKREEP 600
MEEEEEPEQK PCLKPTLRPI SSAPSVSSAS GNATPNTPGD ESPCGIIIPH ENSPDQQQPE 660
EHRPKIGLSL KLGASNSPGQ PNSVKRKKLP VDSVFNKFED EDSDDVPRKR KLVPLDYGED 720
DKNATKGTVN TEEKRKHIKS LIEKIPTAKP ELFAYPLDWS IVDSILMERR IRPWINKKII 780
EYIGEEEATL VDFVCSKVMA HSSPQSILDD VAMVLDEEAE VFIVKMWRLL IYETEAKKIG 840
LVK 843 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0003729; F:mRNA binding; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0097194; P:execution phase of apoptosis; IDA:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IDA:UniProtKB.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR002483; PWI_dom.
 IPR000504; RRM_dom. 
Pfam
 PF01480; PWI
 PF00076; RRM_1 
SMART
 SM00311; PWI
 SM00360; RRM 
PROSITE
 PS51025; PWI
 PS50102; RRM 
PRINTS