CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021227
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrator complex subunit 2 
Protein Synonyms/Alias
 Int2 
Gene Name
 INTS2 
Gene Synonyms/Alias
 KIAA1287 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
69QSWAQDKKLILRLLSubiquitination[1]
108KEQQLRHKLGGGSGEubiquitination[1, 2]
231EVCRGLIKNGERQDEubiquitination[1]
255DALRFLCKMNPSQALubiquitination[1]
263MNPSQALKVRGMVVEubiquitination[1, 2]
574TKHKVSIKDWIYRQLubiquitination[1, 2]
675TLAAMQRKPKSYSSSubiquitination[1]
677AAMQRKPKSYSSSLMubiquitination[1]
690LMDQIPIKFLIRQAQubiquitination[1]
750MLLTNNAKNHSPKQLubiquitination[1]
847IKFVRQQKYTQNDLMubiquitination[1, 2]
897AYLSAHLKETEQDRPubiquitination[1]
977ITTSAPNKGMEEGEDubiquitination[1]
1154ITRLQQIKEKPSGWSubiquitination[1, 3]
1156RLQQIKEKPSGWSQIubiquitination[1, 3]
1165SGWSQICKDSSYKNGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. 
Sequence Annotation
  
Keyword
 Complete proteome; Membrane; Nucleus; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1204 AA 
Protein Sequence
MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ 60
SQSWAQDKKL ILRLLSGVEA VNSIVALLSV DFHALEQDAS KEQQLRHKLG GGSGESILVS 120
QLQHGLTLEF EHSDSPRRLR LVLSELLAIM NKVSESNGEF FFKSSELFES PVYLEEAADV 180
LCILQAELPS LLPIVDVAEA LLHVRNGAWF LCLLVANVPD SFNEVCRGLI KNGERQDEES 240
LGGRRRTDAL RFLCKMNPSQ ALKVRGMVVE ECHLPGLGVA LTLDHTKNEA CEDGVSDLVC 300
FVSGLLLGTN AKVRTWFGTF IRNGQQRKRE TSSSVLWQMR RQLLLELMGI LPTVRSTRIV 360
EEADVDMEPN VSVYSGLKEE HVVKASALLR LYCALMGIAG LKPTEEEAEQ LLQLMTSRPP 420
ATPAGVRFVS LSFCMLLAFS TLVSTPEQEQ LMVVWLSWMI KEEAYFESTS GVSASFGEML 480
LLVAMYFHSN QLSAIIDLVC STLGMKIVIK PSSLSRMKTI FTQEIFTEQV VTAHAVRVPV 540
TSNLSANITG FLPIHCIYQL LRSRSFTKHK VSIKDWIYRQ LCETSTPLHP QLLPLIDVYI 600
NSILTPASKS NPEATNQPVT EQEILNIFQG VIGGDNIRLN QRFSITAQLL VLYYILSYEE 660
ALLANTKTLA AMQRKPKSYS SSLMDQIPIK FLIRQAQGLQ QELGGLHSAL LRLLATNYPH 720
LCIVDDWICE EEITGTDALL RRMLLTNNAK NHSPKQLQEA FSAVPVNNTQ VMQIIEHLTL 780
LSASELIPYA EVLTSNMSQL LNSGVPRRIL QTVNKLWMVL NTVMPRRLWV MTVNALQPSI 840
KFVRQQKYTQ NDLMIDPLIV LRCDQRVHRC PPLMDITLHM LNGYLLASKA YLSAHLKETE 900
QDRPSQNNTI GLVGQTDAPE VTREELKNAL LAAQDSAAVQ ILLEICLPTE EEKANGVNPD 960
SLLRNVQSVI TTSAPNKGME EGEDNLLCNL REVQCLICCL LHQMYIADPN IAKLVHFQGY 1020
PCELLPLTVA GIPSMHICLD FIPELIAQPE LEKQIFAIQL LSHLCIQYAL PKSLSVARLA 1080
VNVMGTLLTV LTQAKRYAFF MPTLPSLVSF CRAFPPLYED IMSLLIQIGQ VCASDVATQT 1140
RDIDPIITRL QQIKEKPSGW SQICKDSSYK NGSRDTGSMD PDVQLCHCIE RTVIEIINMS 1200
VSGI 1204 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0032039; C:integrator complex; IDA:HGNC.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0016180; P:snRNA processing; IDA:HGNC. 
Interpro
 IPR026236; Int2. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR02105; INTSUBUNIT2.