CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004878
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA mismatch repair protein Msh3 
Protein Synonyms/Alias
 hMSH3; Divergent upstream protein; DUP; Mismatch repair protein 1; MRP1 
Gene Name
 MSH3 
Gene Synonyms/Alias
 DUC1; DUG 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103PVKKKVKKVQQKEGGacetylation[1]
107KVKKVQQKEGGSDLGubiquitination[2]
165ERFAVLPKCTDFDDIubiquitination[3]
240ELQYIEMKQQHKDAVubiquitination[3]
244IEMKQQHKDAVLCVEubiquitination[3]
308GYKVGVVKQTETAALubiquitination[3]
316QTETAALKAIGDNRSubiquitination[4]
557NQTDMKTKGSLLWVLubiquitination[3]
586WVTQPLLKLREINARubiquitination[3]
770CIPTDWVKVGSTKAVubiquitination[3]
842FSLAKVAKQGDYCRPubiquitination[3]
902TGPNMGGKSSYIKQVubiquitination[3]
1074SYGLNVAKLADVPGEubiquitination[3]
1085VPGEILKKAAHKSKEubiquitination[3]
1091KKAAHKSKELEGLINubiquitination[3, 4]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion- deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. 
Sequence Annotation
 NP_BIND 896 903 ATP (Potential).
 REGION 75 297 Interaction with EXO1.
 MOD_RES 1099 1099 Phosphothreonine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1137 AA 
Protein Sequence
MSRRKPASGG LAASSSAPAR QAVLSRFFQS TGSLKSTSSS TGAADQVDPG AAAAAAAAAA 60
AAPPAPPAPA FPPQLPPHIA TEIDRRKKRP LENDGPVKKK VKKVQQKEGG SDLGMSGNSE 120
PKKCLRTRNV SKSLEKLKEF CCDSALPQSR VQTESLQERF AVLPKCTDFD DISLLHAKNA 180
VSSEDSKRQI NQKDTTLFDL SQFGSSNTSH ENLQKTASKS ANKRSKSIYT PLELQYIEMK 240
QQHKDAVLCV ECGYKYRFFG EDAEIAAREL NIYCHLDHNF MTASIPTHRL FVHVRRLVAK 300
GYKVGVVKQT ETAALKAIGD NRSSLFSRKL TALYTKSTLI GEDVNPLIKL DDAVNVDEIM 360
TDTSTSYLLC ISENKENVRD KKKGNIFIGI VGVQPATGEV VFDSFQDSAS RSELETRMSS 420
LQPVELLLPS ALSEQTEALI HRATSVSVQD DRIRVERMDN IYFEYSHAFQ AVTEFYAKDT 480
VDIKGSQIIS GIVNLEKPVI CSLAAIIKYL KEFNLEKMLS KPENFKQLSS KMEFMTINGT 540
TLRNLEILQN QTDMKTKGSL LWVLDHTKTS FGRRKLKKWV TQPLLKLREI NARLDAVSEV 600
LHSESSVFGQ IENHLRKLPD IERGLCSIYH KKCSTQEFFL IVKTLYHLKS EFQAIIPAVN 660
SHIQSDLLRT VILEIPELLS PVEHYLKILN EQAAKVGDKT ELFKDLSDFP LIKKRKDEIQ 720
GVIDEIRMHL QEIRKILKNP SAQYVTVSGQ EFMIEIKNSA VSCIPTDWVK VGSTKAVSRF 780
HSPFIVENYR HLNQLREQLV LDCSAEWLDF LEKFSEHYHS LCKAVHHLAT VDCIFSLAKV 840
AKQGDYCRPT VQEERKIVIK NGRHPVIDVL LGEQDQYVPN NTDLSEDSER VMIITGPNMG 900
GKSSYIKQVA LITIMAQIGS YVPAEEATIG IVDGIFTRMG AADNIYKGQS TFMEELTDTA 960
EIIRKATSQS LVILDELGRG TSTHDGIAIA YATLEYFIRD VKSLTLFVTH YPPVCELEKN 1020
YSHQVGNYHM GFLVSEDESK LDPGAAEQVP DFVTFLYQIT RGIAARSYGL NVAKLADVPG 1080
EILKKAAHKS KELEGLINTK RKRLKYFAKL WTMHNAQDLQ KWTEEFNMEE TQTSLLH 1137 
Gene Ontology
 GO:0032302; C:MutSbeta complex; IDA:HGNC.
 GO:0000228; C:nuclear chromosome; IBA:RefGenome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019237; F:centromeric DNA binding; IEA:Compara.
 GO:0003684; F:damaged DNA binding; IEA:Compara.
 GO:0008094; F:DNA-dependent ATPase activity; IBA:RefGenome.
 GO:0000406; F:double-strand/single-strand DNA junction binding; IBA:RefGenome.
 GO:0000404; F:loop DNA binding; IBA:RefGenome.
 GO:0000403; F:Y-form DNA binding; IBA:RefGenome.
 GO:0043570; P:maintenance of DNA repeat elements; IMP:HGNC.
 GO:0000710; P:meiotic mismatch repair; IBA:RefGenome.
 GO:0045910; P:negative regulation of DNA recombination; IDA:BHF-UCL.
 GO:0051096; P:positive regulation of helicase activity; IDA:BHF-UCL.
 GO:0007131; P:reciprocal meiotic recombination; IBA:RefGenome.
 GO:0016447; P:somatic recombination of immunoglobulin gene segments; IBA:RefGenome. 
Interpro
 IPR007695; DNA_mismatch_repair_MutS-lik_N.
 IPR000432; DNA_mismatch_repair_MutS_C.
 IPR007861; DNA_mismatch_repair_MutS_clamp.
 IPR007696; DNA_mismatch_repair_MutS_core.
 IPR016151; DNA_mismatch_repair_MutS_N.
 IPR007860; DNA_mmatch_repair_MutS_con_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF01624; MutS_I
 PF05188; MutS_II
 PF05192; MutS_III
 PF05190; MutS_IV
 PF00488; MutS_V 
SMART
 SM00534; MUTSac
 SM00533; MUTSd 
PROSITE
 PS00486; DNA_MISMATCH_REPAIR_2 
PRINTS