CPLM-000781

Genbank Nucleotide ID

U3 small nucleolar RNA-interacting protein 2

Protein Synonyms/Alias

RRP9 homolog; U3 small nucleolar ribonucleoprotein-associated 55 kDa protein; U3 snoRNP-associated 55 kDa protein; U3-55K

Homo sapiens (Human)

Position	Peptide	Type	References
12	AAARKRGKPASGAGA	ubiquitination	[1]
62	SESLAPRKPEEEEEE	ubiquitination	[1]
84	EKKLRLAKLYLEQLR	ubiquitination	[2]
113	DQVAGRLKEDVLEQR	acetylation	[3]
113	DQVAGRLKEDVLEQR	ubiquitination	[2, 4]
129	RLQKLVAKEIQAPAS	ubiquitination	[1, 2, 4, 5, 6, 7]
180	WSVESGRKLHVIPRA	ubiquitination	[2]
355	ALWGLSKKRPLALQR	ubiquitination	[2]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA.

REPEAT 144 183 WD 1.
REPEAT 197 236 WD 2.
REPEAT 239 278 WD 3.
REPEAT 281 320 WD 4.
REPEAT 322 360 WD 5.
REPEAT 374 413 WD 6.
REPEAT 419 460 WD 7.
MOTIF 8 40 Nuclear localization signal (Potential).
MOD_RES 50 50 Phosphoserine.
MOD_RES 51 51 Phosphoserine.
MOD_RES 53 53 Phosphoserine.
MOD_RES 55 55 Phosphoserine.
MOD_RES 57 57 Phosphoserine.

3D-structure; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; rRNA processing; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030532; C:small nuclear ribonucleoprotein complex; TAS:ProtInc.
GO:0005732; C:small nucleolar ribonucleoprotein complex; TAS:ProtInc.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0006364; P:rRNA processing; TAS:ProtInc.

IPR020472; G-protein_beta_WD-40_rep.
IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION

PR00320; GPROTEINBRPT.