UniProt Accession

 GUAA_YEAST; P38625; D6W042 

Genbank Protein ID

 X70397; Z49809; BK006946 

Genbank Nucleotide ID

 CAA49847.1; CAA89932.1; DAA10116.1 

Protein Name

 GMP synthase [glutamine-hydrolyzing] 

Protein Synonyms/Alias

 GMP synthetase; Glutamine amidotransferase 

Gene Name

 GUA1 

Gene Synonyms/Alias

 YMR217W; YM8261.11 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
141	VWMSHGDKLHGLPTG	acetylation	[1]
241	VDSTVASKLMTEAIG	ubiquitination	[2]
371	LLENMKLKLIEPLRE	acetylation	[1]
381	EPLRELFKDEVRHLG	acetylation	[1]
418	RVLGEVTKEQVEIAR	acetylation	[1]
426	EQVEIARKADNIYIE	ubiquitination	[2]
436	NIYIEEIKKAGLYNQ	acetylation	[1]
517	VTYDITSKPPATVEW	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

  

Sequence Annotation

 DOMAIN 13 202 Glutamine amidotransferase type-1.
 DOMAIN 203 400 GMPS ATP-PPase.
 NP_BIND 231 237 ATP (By similarity).
 ACT_SITE 89 89 For GATase activity (By similarity).
 ACT_SITE 176 176 For GATase activity (By similarity).
 ACT_SITE 178 178 For GATase activity (By similarity).  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glutamine amidotransferase; GMP biosynthesis; Ligase; Nucleotide-binding; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 525 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003922; F:GMP synthase (glutamine-hydrolyzing) activity; IMP:SGD.
 GO:0016462; F:pyrophosphatase activity; IEA:InterPro.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0006177; P:GMP biosynthetic process; IMP:SGD. 

Interpro

 IPR017926; GATASE.
 IPR001674; GMP_synth_C.
 IPR004739; GMP_synth_N.
 IPR022955; GMP_synthase.
 IPR025777; GMPS_ATP_PPase_dom.
 IPR022310; NAD/GMP_synthase.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF00117; GATase
 PF00958; GMP_synt_C
 PF02540; NAD_synthase 

SMART

  

PROSITE

 PS51273; GATASE_TYPE_1
 PS51553; GMPS_ATP_PPASE 

PRINTS