CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021363
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol glycan anchor biosynthesis class U protein 
Protein Synonyms/Alias
 Cell division cycle protein 91-like 1; Protein CDC91-like 1; GPI transamidase component PIG-U 
Gene Name
 PIGU 
Gene Synonyms/Alias
 CDC91L1; PSEC0205; UNQ3055/PRO9875 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42VSPLSSWKRVVEGLSubiquitination[1, 2, 3, 4, 5, 6]
111KVVFKKQKLLLELDQubiquitination[2, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the GPI transamidase complex. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI. 
Sequence Annotation
 REGION 269 289 May be involved in recognition of long-  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; GPI-anchor biosynthesis; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 435 AA 
Protein Sequence
MAAPLVLVLV VAVTVRAALF RSSLAEFISE RVEVVSPLSS WKRVVEGLSL LDLGVSPYSG 60
AVFHETPLII YLFHFLIDYA ELVFMITDAL TAIALYFAIQ DFNKVVFKKQ KLLLELDQYA 120
PDVAELIRTP MEMRYIPLKV ALFYLLNPYT ILSCVAKSTC AINNTLIAFF ILTTIKGSAF 180
LSAIFLALAT YQSLYPLTLF VPGLLYLLQR QYIPVKMKSK AFWIFSWEYA MMYVGSLVVI 240
ICLSFFLLSS WDFIPAVYGF ILSVPDLTPN IGLFWYFFAE MFEHFSLFFV CVFQINVFFY 300
TIPLAIKLKE HPIFFMFIQI AVIAIFKSYP TVGDVALYMA FFPVWNHLYR FLRNIFVLTC 360
IIIVCSLLFP VLWHLWIYAG SANSNFFYAI TLTFNVGQIL LISDYFYAFL RREYYLTHGL 420
YLTAKDGTEA MLVLK 435 
Gene Ontology
 GO:0042765; C:GPI-anchor transamidase complex; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HGNC.
 GO:0016255; P:attachment of GPI anchor to protein; IMP:UniProtKB.
 GO:0006501; P:C-terminal protein lipidation; TAS:Reactome.
 GO:0046425; P:regulation of JAK-STAT cascade; IDA:HGNC. 
Interpro
 IPR009600; PIG-U. 
Pfam
 PF06728; PIG-U 
SMART
  
PROSITE
  
PRINTS