CPLM-021962

Genbank Nucleotide ID

Protein Synonyms/Alias

8D6 antigen; FDC-signaling molecule 8D6; FDC-SM-8D6; Transcobalamin receptor; TCblR; CD320

Homo sapiens (Human)

Position	Peptide	Type	References
97	RIEPCTQKGQCPPPP	ubiquitination	[1]
122	DCSGGTDKKLRNCSR	ubiquitination	[1]
123	CSGGTDKKLRNCSRL	ubiquitination	[1]
269	LGLLVAMKESLLLSE	ubiquitination	[1, 2, 3, 4, 5, 6]
278	SLLLSEQKTSLP***	ubiquitination	[1, 2, 3, 5, 6, 7, 8, 9]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] A data set of human endogenous protein ubiquitination sites.
Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
[8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin.

DOMAIN 53 90 LDL-receptor class A 1.
DOMAIN 131 168 LDL-receptor class A 2.
CARBOHYD 126 126 N-linked (GlcNAc...) (Potential).
CARBOHYD 195 195 N-linked (GlcNAc...) (Potential).
CARBOHYD 213 213 N-linked (GlcNAc...) (Potential).
DISULFID 54 67 By similarity.
DISULFID 61 80 By similarity.
DISULFID 74 89 By similarity.
DISULFID 132 145 By similarity.
DISULFID 139 158 By similarity.
DISULFID 152 167 By similarity.

Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Growth factor; Membrane; Polymorphism; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005783; C:endoplasmic reticulum; IDA:LIFEdb.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005886; C:plasma membrane; IDA:MGI.
GO:0031419; F:cobalamin binding; IDA:MGI.
GO:0001558; P:regulation of cell growth; NAS:UniProtKB.

IPR023415; LDLR_class-A_CS.
IPR002172; LDrepeatLR_classA_rpt.

PF00057; Ldl_recept_a

PS01209; LDLRA_1
PS50068; LDLRA_2