UniProt Accession

 KLH20_HUMAN; Q9Y2M5; B3KMA0; Q5TZF2; Q5ZF45; Q9H457 

Genbank Protein ID

 AJ844466; AB026190; AK001430; AL109921; BX248409; BX248409; AL109921; BC063418 

Genbank Nucleotide ID

 CAH59617.1; BAA77027.1; BAG50912.1; CAI20377.1; CAI20377.1; CAH73000.1; CAH73000.1; AAH63418.1 

Protein Name

 Kelch-like protein 20 

Protein Synonyms/Alias

 Kelch-like ECT2-interacting protein; Kelch-like protein X 

Gene Name

 KLHL20 

Gene Synonyms/Alias

 KLEIP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
265	RLPLLSPKFLVGTVG	ubiquitination	[1, 2, 3]
388	SVERYDPKTNQWSSD	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. 

Sequence Annotation

 DOMAIN 68 135 BTB.
 DOMAIN 170 272 BACK.
 REPEAT 319 365 Kelch 1.
 REPEAT 367 413 Kelch 2.
 REPEAT 414 460 Kelch 3.
 REPEAT 462 507 Kelch 4.
 REPEAT 509 554 Kelch 5.
 REPEAT 556 601 Kelch 6.  

Keyword

 Actin-binding; Complete proteome; Cytoplasm; Kelch repeat; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 609 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; TAS:ProtInc.
 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016605; C:PML body; IDA:UniProtKB.
 GO:0019964; F:interferon-gamma binding; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0007010; P:cytoskeleton organization; TAS:ProtInc.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0035455; P:response to interferon-alpha; TAS:UniProtKB. 

Interpro

 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 

Pfam

 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 

SMART

 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 

PROSITE

 PS50097; BTB 

PRINTS