UniProt Accession

 RGN_RAT; Q03336; Q63496; Q925W3; Q9QWP2 

Genbank Protein ID

 X69021; D38467; AB037934; BC078794; D67070; D31662 

Genbank Nucleotide ID

 CAA48786.1; BAA07490.1; BAA90692.1; AAH78794.1; BAA11083.1; BAA06507.1 

Protein Name

 Regucalcin 

Protein Synonyms/Alias

 RC; Gluconolactonase; GNL; Senescence marker protein 30; SMP-30 

Gene Name

 Rgn 

Gene Synonyms/Alias

 Smp30 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
144	LFPDHSVKKYFDQVD	acetylation	[1]
145	FPDHSVKKYFDQVDI	acetylation	[1]
195	SNRRTVYKMEKDEQI	acetylation	[1]
233	RLDPETGKRLQTVKL	acetylation	[1]
239	GKRLQTVKLPVDKTT	acetylation	[1]
283	PDAGNIFKITGLGVK	acetylation	[1]
290	KITGLGVKGIAPYSY	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities. 

Sequence Annotation

 ACT_SITE 204 204 Proton donor/acceptor (By similarity).
 METAL 18 18 Divalent metal cation (By similarity).
 METAL 154 154 Divalent metal cation (By similarity).
 METAL 204 204 Divalent metal cation (By similarity).
 BINDING 101 101 Substrate (By similarity).
 BINDING 103 103 Substrate (By similarity).
 BINDING 121 121 Substrate (By similarity).
 MOD_RES 3 3 Phosphoserine (By similarity).  

Keyword

 Ascorbate biosynthesis; Calcium; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 299 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0030234; F:enzyme regulator activity; IEA:InterPro.
 GO:0004341; F:gluconolactonase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; ISS:UniProtKB.
 GO:0006874; P:cellular calcium ion homeostasis; IDA:UniProtKB.
 GO:0019853; P:L-ascorbic acid biosynthetic process; ISS:UniProtKB.
 GO:0032781; P:positive regulation of ATPase activity; IDA:UniProtKB.
 GO:0050848; P:regulation of calcium-mediated signaling; IDA:UniProtKB. 

Interpro

 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR008367; Regucalcin.
 IPR013658; SGL.
 IPR005511; SMP-30. 

Pfam

 PF08450; SGL 

SMART

  

PROSITE

  

PRINTS

 PR01791; REGUCALCIN.
 PR01790; SMP30FAMILY.