CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004271
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UDP-N-acetylmuramoylalanine--D-glutamate ligase 
Protein Synonyms/Alias
 D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase 
Gene Name
 murD 
Gene Synonyms/Alias
 b0088; JW0086 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
46MTPPGLDKLPEAVERacetylation[1]
255WLRVKGEKVLNVKEMacetylation[1]
263VLNVKEMKLSGQHNYacetylation[1]
421CASLDQFKNFEQRGNacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA). 
Sequence Annotation
 NP_BIND 112 118 ATP (Potential).  
Keyword
 3D-structure; ATP-binding; Cell cycle; Cell division; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Peptidoglycan synthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 438 AA 
Protein Sequence
MADYQGKNVV IIGLGLTGLS CVDFFLARGV TPRVMDTRMT PPGLDKLPEA VERHTGSLND 60
EWLMAADLIV ASPGIALAHP SLSAAADAGI EIVGDIELFC REAQAPIVAI TGSNGKSTVT 120
TLVGEMAKAA GVNVGVGGNI GLPALMLLDD ECELYVLELS SFQLETTSSL QAVAATILNV 180
TEDHMDRYPF GLQQYRAAKL RIYENAKVCV VNADDALTMP IRGADERCVS FGVNMGDYHL 240
NHQQGETWLR VKGEKVLNVK EMKLSGQHNY TNALAALALA DAAGLPRASS LKALTTFTGL 300
PHRFEVVLEH NGVRWINDSK ATNVGSTEAA LNGLHVDGTL HLLLGGDGKS ADFSPLARYL 360
NGDNVRLYCF GRDGAQLAAL RPEVAEQTET MEQAMRLLAP RVQPGDMVLL SPACASLDQF 420
KNFEQRGNEF ARLAKELG 438 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0008764; F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity; IDA:EcoCyc.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. 
Interpro
 IPR004101; Mur_ligase_C.
 IPR013221; Mur_ligase_cen.
 IPR016040; NAD(P)-bd_dom.
 IPR005762; UDP-N-AcMur-Glu_ligase. 
Pfam
 PF02875; Mur_ligase_C
 PF08245; Mur_ligase_M 
SMART
  
PROSITE
  
PRINTS