UniProt Accession

 YBOX1_MOUSE; P62960; P22568; P27817; P43482 

Genbank Protein ID

 M60419; M62867; X57621; BC013450; BC013620; BC029747; BC031472; BC061634 

Genbank Nucleotide ID

 AAA40577.1; AAA63390.1; CAA40847.1; AAH13450.1; AAH13620.1; AAH29747.1; AAH31472.1; AAH61634.1 

Protein Name

 Nuclease-sensitive element-binding protein 1 

Protein Synonyms/Alias

 CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1 

Gene Name

 Ybx1 

Gene Synonyms/Alias

 Msy-1; Msy1; Nsep1; Yb1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
56	DKKVIATKVLGTVKW	acetylation	[1]
62	TKVLGTVKWFNVRNG	ubiquitination	[2]
90	FVHQTAIKKNNPRKY	ubiquitination	[2]
91	VHQTAIKKNNPRKYL	ubiquitination	[2]
135	GVPVQGSKYAADRNH	ubiquitination	[2]
168	QNSESGEKNEGSESA	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Mediates pre-mRNA alternative splicing regulation. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. Regulates the transcription of numerous genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds preferentially to 5'-[CU]CUGCG-3' motif in vitro (By similarity). 

Sequence Annotation

 DOMAIN 59 123 CSD.
 REGION 16 69 Interaction with ss-DNA (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 100 100 Phosphoserine; by PKB/AKT1 (By
 MOD_RES 160 160 Phosphotyrosine (By similarity).
 MOD_RES 163 163 Phosphoserine.
 MOD_RES 165 165 Phosphoserine (By similarity).
 MOD_RES 172 172 Phosphoserine.
 MOD_RES 174 174 Phosphoserine.
 MOD_RES 299 299 N6-acetyllysine (By similarity).
 MOD_RES 302 302 N6-acetyllysine (By similarity).
 MOD_RES 312 312 Phosphoserine.
 CROSSLNK 135 135 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Activator; Complete proteome; Cytoplasm; DNA-binding; Isopeptide bond; Mitogen; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repressor; RNA-binding; Secreted; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 322 AA 

Protein Sequence

Gene Ontology

 GO:0070937; C:CRD-mediated mRNA stability complex; IEA:Compara.
 GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0071204; C:histone pre-mRNA 3'end processing complex; IDA:UniProtKB.
 GO:0005689; C:U12-type spliceosomal complex; IEA:Compara.
 GO:0003723; F:RNA binding; ISS:UniProtKB.
 GO:0003697; F:single-stranded DNA binding; IDA:MGI.
 GO:0070934; P:CRD-mediated mRNA stabilization; ISS:UniProtKB.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0051154; P:negative regulation of striated muscle cell differentiation; IDA:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR019844; Cold-shock_CS.
 IPR011129; Cold_shock_prot.
 IPR002059; CSP_DNA-bd.
 IPR012340; NA-bd_OB-fold. 

Pfam

 PF00313; CSD 

SMART

 SM00357; CSP 

PROSITE

 PS00352; COLD_SHOCK 

PRINTS

 PR00050; COLDSHOCK.