UniProt Accession

 SPC19_YEAST; Q03954; D6VSI3 

Genbank Protein ID

 Z48784; AY557667; BK006938 

Genbank Nucleotide ID

 CAA88713.1; AAS55993.1; DAA12043.1 

Protein Name

 DASH complex subunit SPC19 

Protein Synonyms/Alias

 19 kDa spindle pole component protein; Outer kinetochore protein SPC19 

Gene Name

 SPC19 

Gene Synonyms/Alias

 YDR201W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
44	ASTMLQTKRVFRLVP	ubiquitination	[1]
158	KKKQLENKLEILKQK	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. 

Sequence Annotation

 MOD_RES 107 107 Phosphoserine.
 MOD_RES 116 116 Phosphoserine.  

Keyword

 Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 165 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0042729; C:DASH complex; IDA:SGD.
 GO:0005876; C:spindle microtubule; IDA:SGD.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005200; F:structural constituent of cytoskeleton; IPI:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007020; P:microtubule nucleation; IPI:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0030472; P:mitotic spindle organization in nucleus; IPI:SGD.
 GO:0031110; P:regulation of microtubule polymerization or depolymerization; IPI:SGD. 

Interpro

 IPR013251; DASH_Spc19. 

Pfam

 PF08287; DASH_Spc19 

SMART

  

PROSITE

  

PRINTS