CPLM-008732

Genbank Nucleotide ID

AP-2 complex subunit sigma

Protein Synonyms/Alias

Adapter-related protein complex 2 sigma subunit; Adaptor protein complex AP-2 subunit sigma; Clathrin assembly protein 2 small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; Plasma membrane adaptor AP-2 17 kDa protein; Sigma2-adaptin

Homo sapiens (Human)

Position	Peptide	Type	References
18	AGKTRLAKWYMQFDD	ubiquitination	[1, 2, 3, 4, 5, 6]
28	MQFDDDEKQKLIEEV	ubiquitination	[1, 2, 4, 5, 6]
30	FDDDEKQKLIEEVHA	ubiquitination	[4]
45	VVTVRDAKHTNFVEF	ubiquitination	[2, 3, 4, 5, 7, 8]
56	FVEFRNFKIIYRRYA	ubiquitination	[1, 2, 5, 6]
130	IRETSQTKVLKQLLM	ubiquitination	[1, 2, 3, 4, 5, 6]
133	TSQTKVLKQLLMLQS	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). May also play a role in extracellular calcium homeostasis.

MOD_RES 140 140 Phosphoserine.

Alternative splicing; Cell membrane; Coated pit; Complete proteome; Disease mutation; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030122; C:AP-2 adaptor complex; TAS:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0008565; F:protein transporter activity; NAS:UniProtKB.
GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
GO:0007411; P:axon guidance; TAS:Reactome.
GO:0048268; P:clathrin coat assembly; TAS:UniProtKB.
GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
GO:0006886; P:intracellular protein transport; IEA:InterPro.
GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome.
GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome.
GO:0030100; P:regulation of endocytosis; TAS:UniProtKB.
GO:0007268; P:synaptic transmission; TAS:Reactome.
GO:0016032; P:viral reproduction; TAS:Reactome.

IPR016635; AP_complex_ssu.
IPR022775; AP_mu_sigma_su.
IPR027156; APS2.
IPR000804; Clathrin_sm-chain_CS.
IPR011012; Longin-like_dom.

PF01217; Clat_adaptor_s

PS00989; CLAT_ADAPTOR_S