CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010823
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor E2F1 
Protein Synonyms/Alias
 E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1 
Gene Name
 E2F1 
Gene Synonyms/Alias
 RBBP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
117GRGRHPGKGVKSPGEacetylation[1, 2]
120RHPGKGVKSPGEKSRacetylation[1, 2]
125GVKSPGEKSRYETSLacetylation[1, 2]
185QLIAKKSKNHIQWLGmethylation[3]
Reference
 [1] Regulation of E2F1 activity by acetylation.
 Martínez-Balbás MA, Bauer UM, Nielsen SJ, Brehm A, Kouzarides T.
 EMBO J. 2000 Feb 15;19(4):662-71. [PMID: 10675335]
 [2] E2F family members are differentially regulated by reversible acetylation.
 Marzio G, Wagener C, Gutierrez MI, Cartwright P, Helin K, Giacca M.
 J Biol Chem. 2000 Apr 14;275(15):10887-92. [PMID: 10753885]
 [3] Lysine methylation regulates E2F1-induced cell death.
 Kontaki H, Talianidis I.
 Mol Cell. 2010 Jul 9;39(1):152-60. [PMID: 20603083] 
Functional Description
 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. 
Sequence Annotation
 DNA_BIND 110 194 Potential.
 REGION 67 108 Cyclin A/CDK2 binding.
 REGION 89 191 Interaction with BIRC2/c-IAP1.
 REGION 153 174 Leucine-zipper.
 REGION 192 382 Required for interaction with TRIM28.
 REGION 195 284 Dimerization (Potential).
 REGION 368 437 Transactivation.
 REGION 409 426 Retinoblastoma protein RB1 binding
 MOTIF 158 194 DEF box.
 MOD_RES 117 117 N6-acetyllysine.
 MOD_RES 120 120 N6-acetyllysine.
 MOD_RES 125 125 N6-acetyllysine.
 MOD_RES 364 364 Phosphoserine; by CHEK2 (Probable).
 MOD_RES 375 375 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Activator; Apoptosis; Cell cycle; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 437 AA 
Protein Sequence
MALAGAPAGG PCAPALEALL GAGALRLLDS SQIVIISAAQ DASAPPAPTG PAAPAAGPCD 60
PDLLLFATPQ APRPTPSAPR PALGRPPVKR RLDLETDHQY LAESSGPARG RGRHPGKGVK 120
SPGEKSRYET SLNLTTKRFL ELLSHSADGV VDLNWAAEVL KVQKRRIYDI TNVLEGIQLI 180
AKKSKNHIQW LGSHTTVGVG GRLEGLTQDL RQLQESEQQL DHLMNICTTQ LRLLSEDTDS 240
QRLAYVTCQD LRSIADPAEQ MVMVIKAPPE TQLQAVDSSE NFQISLKSKQ GPIDVFLCPE 300
ETVGGISPGK TPSQEVTSEE ENRATDSATI VSPPPSSPPS SLTTDPSQSL LSLEQEPLLS 360
RMGSLRAPVD EDRLSPLVAA DSLLEHVRED FSGLLPEEFI SLSPPHEALD YHFGLEEGEG 420
IRDLFDCDFG DLTPLDF 437 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0035189; C:Rb-E2F complex; IDA:BHF-UCL.
 GO:0001047; F:core promoter binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0071398; P:cellular response to fatty acid; IEA:Compara.
 GO:0071456; P:cellular response to hypoxia; IEA:Compara.
 GO:0000077; P:DNA damage checkpoint; IMP:UniProtKB.
 GO:0030900; P:forebrain development; IEA:Compara.
 GO:0000080; P:G1 phase of mitotic cell cycle; TAS:ProtInc.
 GO:0000085; P:G2 phase of mitotic cell cycle; TAS:Reactome.
 GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IMP:UniProtKB.
 GO:0048255; P:mRNA stabilization; IDA:BHF-UCL.
 GO:0071930; P:negative regulation of transcription involved in G1/S phase of mitotic cell cycle; IMP:BHF-UCL.
 GO:0048146; P:positive regulation of fibroblast proliferation; IMP:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; IMP:BHF-UCL.
 GO:0007283; P:spermatogenesis; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR015633; E2F.
 IPR003316; E2F_TDP.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF02319; E2F_TDP 
SMART
  
PROSITE
  
PRINTS