UniProt Accession

 OTU1_MOUSE; Q8CB27; B2RSW9; Q8BPM9; Q8CB24 

Genbank Protein ID

 AK036938; AK036991; AK053730; BC139034 

Genbank Nucleotide ID

 BAC29646.1; BAC29661.1; BAC35495.1; AAI39035.1 

Protein Name

 Ubiquitin thioesterase OTU1 

Protein Synonyms/Alias

  

Gene Name

 Yod1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
49	WRVRCKAKGGTHLLQ	ubiquitination	[1]
130	KASPAFSKYGAPSYV	ubiquitination	[1]
252	GEDAGYTKRVLLIYD	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin (By similarity). 

Sequence Annotation

 DOMAIN 144 269 OTU.
 ZN_FING 313 337 C2H2-type.
 REGION 45 123 UBX-like.
 ACT_SITE 152 152 By similarity.
 ACT_SITE 155 155 Nucleophile (By similarity).
 ACT_SITE 337 337 By similarity.  

Keyword

 3D-structure; Complete proteome; Hydrolase; Metal-binding; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway; Unfolded protein response; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 343 AA 

Protein Sequence

Gene Ontology

 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006520; P:cellular amino acid metabolic process; ISS:UniProtKB.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; ISS:UniProtKB.
 GO:0030433; P:ER-associated protein catabolic process; ISS:UniProtKB.
 GO:0071108; P:protein K48-linked deubiquitination; ISS:UniProtKB.
 GO:0070536; P:protein K63-linked deubiquitination; ISS:UniProtKB. 

Interpro

 IPR003323; OTU.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 

Pfam

 PF02338; OTU 

SMART

 SM00355; ZnF_C2H2 

PROSITE

 PS50802; OTU
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 

PRINTS