UniProt Accession

 CRY2_HUMAN; Q49AN0; B4DH32; O75148; Q8IV71 

Genbank Protein ID

 AK294904; AC068385; BC035161; BC041814; AB014558 

Genbank Nucleotide ID

 BAG57993.1; AAH35161.1; AAH41814.1; BAA31633.1 

Protein Name

 Cryptochrome-2 

Protein Synonyms/Alias

  

Gene Name

 CRY2 

Gene Synonyms/Alias

 KIAA0658 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
126	YDSEPFGKERDAAIM	ubiquitination	[1]
184	SRMELPKKPVGLVTS	ubiquitination	[1]
242	EALARLDKHLERKAW	ubiquitination	[2]
247	LDKHLERKAWVANYE	ubiquitination	[1]
475	NAPESIQKAAKCIIG	ubiquitination	[1, 3]
478	ESIQKAAKCIIGVDY	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. 

Sequence Annotation

 DOMAIN 22 151 Photolyase/cryptochrome alpha/beta.
 REGION 230 507 FAD-binding.
 REGION 390 489 Required for inhibition of CLOCK-ARNTL-
 MOD_RES 266 266 Phosphoserine; by MAPK (By similarity).
 MOD_RES 554 554 Phosphoserine; by GSK3-beta (By
 MOD_RES 558 558 Phosphoserine; by DYRK1A and MAPK (By
 CROSSLNK 126 126 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 242 242 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 348 348 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 475 475 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 504 504 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Alternative splicing; Biological rhythms; Chromophore; Complete proteome; Cytoplasm; FAD; Flavoprotein; Isopeptide bond; Nucleotide-binding; Nucleus; Phosphoprotein; Photoreceptor protein; Receptor; Reference proteome; Repressor; Sensory transduction; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 593 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005576; C:extracellular region; IDA:MGI.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0009882; F:blue light photoreceptor activity; NAS:UniProtKB.
 GO:0003684; F:damaged DNA binding; IDA:UniProtKB.
 GO:0003913; F:DNA photolyase activity; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0000988; F:protein binding transcription factor activity; IDA:BHF-UCL.
 GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
 GO:0007623; P:circadian rhythm; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:InterPro.
 GO:0032515; P:negative regulation of phosphoprotein phosphatase activity; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0018298; P:protein-chromophore linkage; IEA:UniProtKB-KW.
 GO:2000118; P:regulation of sodium-dependent phosphate transport; IDA:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR006050; DNA_photolyase_N.
 IPR005101; Photolyase_FAD-bd/Cryptochr_C.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF00875; DNA_photolyase
 PF03441; FAD_binding_7 

SMART

  

PROSITE

 PS00394; DNA_PHOTOLYASES_1_1
 PS51645; PHR_CRY_ALPHA_BETA 

PRINTS