UniProt Accession

 D4AEP0_RAT; D4AEP0 

Genbank Protein ID

 AABR06076324; CH473985 

Genbank Nucleotide ID

 EDL94793.1 

Protein Name

 Adenylosuccinate synthetase isozyme 2 

Protein Synonyms/Alias

 AMPSase 2; AdSS 2; Adenylosuccinate synthetase, acidic isozyme; Adenylosuccinate synthetase, liver isozyme; IMP--aspartate ligase 2 

Gene Name

 Adss 

Gene Synonyms/Alias

 Adss2; Adss_predicted; rCG_20425 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
419	ISNARTFKELPVNAQ	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP (By similarity). 

Sequence Annotation

 NP_BIND 39 45 GTP (By similarity).
 NP_BIND 67 69 GTP (By similarity).
 NP_BIND 362 364 GTP (By similarity).
 NP_BIND 444 447 GTP (By similarity).
 REGION 40 43 IMP binding (By similarity).
 REGION 65 68 IMP binding (By similarity).
 REGION 330 336 Substrate binding (By similarity).
 ACT_SITE 40 40 Proton acceptor (By similarity).
 ACT_SITE 68 68 Proton donor (By similarity).
 METAL 40 40 Magnesium (By similarity).
 METAL 67 67 Magnesium; via carbonyl oxygen (By
 BINDING 40 40 Substrate (By similarity).
 BINDING 162 162 IMP (By similarity).
 BINDING 176 176 IMP; shared with dimeric partner (By
 BINDING 255 255 IMP (By similarity).
 BINDING 270 270 IMP (By similarity).
 BINDING 334 334 IMP (By similarity).
 BINDING 336 336 GTP (By similarity).  

Keyword

 Complete proteome; Cytoplasm; GTP-binding; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 456 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0004019; F:adenylosuccinate synthase activity; IDA:RGD.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0044208; P:'de novo' AMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006531; P:aspartate metabolic process; IDA:RGD.
 GO:0071257; P:cellular response to electrical stimulus; IEP:RGD.
 GO:0006184; P:GTP catabolic process; IDA:RGD.
 GO:0046040; P:IMP metabolic process; IDA:RGD.
 GO:0060359; P:response to ammonium ion; IEP:RGD.
 GO:0014074; P:response to purine-containing compound; IEP:RGD. 

Interpro

 IPR018220; Adenylosuccinate_synthase_AS.
 IPR001114; Adenylosuccinate_synthetase.
 IPR027529; AdSS_2_vert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00709; Adenylsucc_synt 

SMART

 SM00788; Adenylsucc_synt 

PROSITE

 PS01266; ADENYLOSUCCIN_SYN_1
 PS00513; ADENYLOSUCCIN_SYN_2 

PRINTS