CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014744
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrator complex subunit 3 
Protein Synonyms/Alias
 Int3; SOSS complex subunit A; Sensor of single-strand DNA complex subunit A; SOSS-A; Sensor of ssDNA subunit A 
Gene Name
 INTS3 
Gene Synonyms/Alias
 C1orf193; C1orf60 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MELQKGKGAAAAAAubiquitination[1, 2]
859KPSEEMVKMVLSRPCubiquitination[3, 4]
898HIKSLLIKNNSLPRKubiquitination[3, 4, 5]
915SLRSSSSKLAQLTLEubiquitination[1]
939RLNLTNTKQNFFSQTubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Component of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. 
Sequence Annotation
 MOD_RES 502 502 Phosphoserine.
 MOD_RES 537 537 Phosphoserine.
 MOD_RES 995 995 Phosphoserine.
 MOD_RES 1040 1040 Phosphoserine (By similarity).
 MOD_RES 1042 1042 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1043 AA 
Protein Sequence
MELQKGKGAA AAAAASGAAG GGGGGAGAGA PGGGRLLLST SLDAKDELEE RLERCMSIVT 60
SMTAGVSERE ANDALNAYVC KGLPQHEEIC LGLFTLILTE PAQAQKCYRD LALVSRDGMN 120
IVLNKINQIL MEKYLKLQDT CRTQLVWLVR ELVKSGVLGA DGVCMTFMKQ IAGGGDVTAK 180
NIWLAESVLD ILTEQREWVL KSSILIAMAV YTYLRLIVDH HGTAQLQALR QKEVDFCISL 240
LRERFMECLM IGRDLVRLLQ NVARIPEFEL LWKDIIHNPQ ALSPQFTGIL QLLQSRTSRK 300
FLACRLTPDM ETKLLFMTSR VRFGQQKRYQ DWFQRQYLST PDSQSLRCDL IRYICGVVHP 360
SNEVLSSDIL PRWAIIGWLL TTCTSNVAAS NAKLALFYDW LFFSPDKDSI MNIEPAILVM 420
HHSMKPHPAI TATLLDFMCR IIPNFYPPLE GHVRQGVFSS LNHIVEKRVL AHLAPLFDNP 480
KLDKELRAML REKFPEFCSS PSPPVEVKIE EPVSMEMDNH MSDKDESCYD NAEAAFSDDE 540
EDLNSKGKKR EFRFHPIKET VVEEPVDITP YLDQLDESLR DKVLQLQKGS DTEAQCEVMQ 600
EIVDQVLEED FDSEQLSVLA SCLQELFKAH FRGEVLPEEI TEESLEESVG KPLYLIFRNL 660
CQMQEDNSSF SLLLDLLSEL YQKQPKIGYH LLYYLRASKA AAGKMNLYES FAQATQLGDL 720
HTCLMMDMKA CQEDDVRLLC HLTPSIYTEF PDETLRSGEL LNMIVAVIDS AQLQELVCHV 780
MMGNLVMFRK DSVLNILIQS LDWETFEQYC AWQLFLAHNI PLETIIPILQ HLKYKEHPEA 840
LSCLLLQLRR EKPSEEMVKM VLSRPCHPDD QFTTSILRHW CMKHDELLAE HIKSLLIKNN 900
SLPRKRQSLR SSSSKLAQLT LEQILEHLDN LRLNLTNTKQ NFFSQTPILQ ALQHVQASCD 960
EAHKMKFSDL FSLAEEYEDS STKPPKSRRK AALSSPRSRK NATQPPNAEE ESGSSSASEE 1020
EDTKPKPTKR KRKGSSAVGS DSD 1043 
Gene Ontology
 GO:0032039; C:integrator complex; IDA:HGNC.
 GO:0070876; C:SOSS complex; IDA:UniProtKB.
 GO:0000075; P:cell cycle checkpoint; IMP:UniProtKB.
 GO:0006281; P:DNA repair; IMP:UniProtKB.
 GO:0010212; P:response to ionizing radiation; IMP:UniProtKB.
 GO:0016180; P:snRNA processing; IDA:HGNC. 
Interpro
 IPR019333; Int_cplx_su3. 
Pfam
 PF10189; DUF2356 
SMART
  
PROSITE
  
PRINTS