UniProt Accession

 EAF3_YEAST; Q12432; D6W433 

Genbank Protein ID

 Z71255; Z49274; BK006949 

Genbank Nucleotide ID

 CAA95019.1; CAA89277.1; DAA11449.1 

Protein Name

 Chromatin modification-related protein EAF3 

Protein Synonyms/Alias

 ESA1-associated factor 3 

Gene Name

 EAF3 

Gene Synonyms/Alias

 YPR023C; YP9367.03C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
45	YTSIPNDKPGGSSQA	acetylation	[1]
54	GGSSQATKEIKPQKL	acetylation	[1]
156	RSNASISKSTSQSFL	acetylation	[1, 2]

Reference

 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. 

Sequence Annotation

 DOMAIN 216 399 MRG.
 MOD_RES 201 201 Phosphoserine.  

Keyword

 3D-structure; Chromatin regulator; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 401 AA 

Protein Sequence

Gene Ontology

 GO:0035267; C:NuA4 histone acetyltransferase complex; IPI:SGD.
 GO:0033698; C:Rpd3L complex; IDA:SGD.
 GO:0032221; C:Rpd3S complex; IDA:SGD.
 GO:0004402; F:histone acetyltransferase activity; IDA:SGD.
 GO:0006281; P:DNA repair; IDA:SGD.
 GO:0016575; P:histone deacetylation; IMP:SGD.
 GO:0060195; P:negative regulation of antisense RNA transcription; IMP:SGD.
 GO:0030174; P:regulation of DNA-dependent DNA replication initiation; IMP:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IGI:SGD. 

Interpro

 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR008676; MRG.
 IPR026541; MRG_dom.
 IPR025995; Tudor-knot. 

Pfam

 PF05712; MRG
 PF11717; Tudor-knot 

SMART

 SM00298; CHROMO 

PROSITE

 PS51640; MRG 

PRINTS