UniProt Accession

 P4HTM_HUMAN; Q9NXG6; Q6PAG6; Q8TCJ9; Q8WV55; Q96F22; Q9BW77 

Genbank Protein ID

 AY198406; AL713728; BC000580; BC011710; BC047566; BC060321; AK000269 

Genbank Nucleotide ID

 AAO43431.1; CAD28518.2; AAH00580.1; AAH11710.3; AAH47566.1; AAH60321.1; BAA91045.1 

Protein Name

 Transmembrane prolyl 4-hydroxylase 

Protein Synonyms/Alias

 P4H-TM; Hypoxia-inducible factor prolyl hydroxylase 4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4 

Gene Name

 P4HTM 

Gene Synonyms/Alias

 PH4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
141	FIRTLSLKPLLFEIP	ubiquitination	[1]
265	HKYMRSHKAESSELV	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. 

Sequence Annotation

 DOMAIN 185 220 EF-hand 1.
 DOMAIN 224 259 EF-hand 2.
 DOMAIN 310 460 Fe2OG dioxygenase.
 METAL 328 328 Iron (By similarity).
 METAL 330 330 Iron (By similarity).
 METAL 374 374 Iron (By similarity).
 BINDING 451 451 2-oxoglutarate (Potential).
 CARBOHYD 348 348 N-linked (GlcNAc...) (Potential).
 CARBOHYD 368 368 N-linked (GlcNAc...) (Potential).
 CARBOHYD 382 382 N-linked (GlcNAc...) (Potential).  

Keyword

 Alternative splicing; Calcium; Complete proteome; Dioxygenase; Endoplasmic reticulum; Glycoprotein; Iron; Membrane; Metal-binding; Oxidoreductase; Reference proteome; Repeat; Signal-anchor; Transmembrane; Transmembrane helix; Vitamin C. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 502 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
 GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; IEA:InterPro.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR005123; Oxoglu/Fe-dep_dioxygenase.
 IPR006620; Pro_4_hyd_alph. 

Pfam

 PF13640; 2OG-FeII_Oxy_3
 PF13202; EF_hand_3 

SMART

 SM00702; P4Hc 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51471; FE2OG_OXY 

PRINTS