CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015741
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho-related GTP-binding protein RhoU 
Protein Synonyms/Alias
 CDC42-like GTPase 1; GTP-binding protein-like 1; Rho GTPase-like protein ARHU; Ryu GTPase; Wnt-1 responsive Cdc42 homolog 1; WRCH-1 
Gene Name
 RHOU 
Gene Synonyms/Alias
 ARHU; CDC42L1; G28K; WRCH1; SB128 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
196KLCAEEIKAASYIECubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP- bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. 
Sequence Annotation
 NP_BIND 56 63 GTP.
 NP_BIND 103 107 GTP (By similarity).
 NP_BIND 161 164 GTP.
 LIPID 256 256 S-palmitoyl cysteine.  
Keyword
 3D-structure; Alternative splicing; Cell junction; Cell membrane; Cell projection; Complete proteome; Golgi apparatus; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Palmitate; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 258 AA 
Protein Sequence
MPPQQGDPAF PDRCEAPPVP PRRERGGRGG RGPGEPGGRG RAGGAEGRGV KCVLVGDGAV 60
GKTSLVVSYT TNGYPTEYIP TAFDNFSAVV SVDGRPVRLQ LCDTAGQDEF DKLRPLCYTN 120
TDIFLLCFSV VSPSSFQNVS EKWVPEIRCH CPKAPIILVG TQSDLREDVK VLIELDKCKE 180
KPVPEEAAKL CAEEIKAASY IECSALTQKN LKEVFDAAIV AGIQYSDTQQ QPKKSKSRTP 240
DKMKNLSKSW WKKYCCFV 258 
Gene Ontology
 GO:0042995; C:cell projection; IEA:UniProtKB-KW.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0002102; C:podosome; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0030036; P:actin cytoskeleton organization; IEA:Compara.
 GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0016601; P:Rac protein signal transduction; IEA:Compara.
 GO:0008360; P:regulation of cell shape; IMP:UniProtKB.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 
Pfam
 PF00071; Ras 
SMART
 SM00174; RHO 
PROSITE
 PS51420; RHO 
PRINTS
 PR00449; RASTRNSFRMNG.