CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002614
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 4F2 cell-surface antigen heavy chain 
Protein Synonyms/Alias
 4F2hc; 4F2 heavy chain antigen; Lymphocyte activation antigen 4F2 large subunit; CD98 
Gene Name
 SLC3A2 
Gene Synonyms/Alias
 MDU1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
111QDTEVDMKEVELNELubiquitination[1, 2]
122LNELEPEKQPMNAASubiquitination[1, 2]
140MSLAGAEKNGLVKIKubiquitination[1, 2]
145AEKNGLVKIKVAEDEubiquitination[1, 2, 3]
147KNGLVKIKVAEDEAEubiquitination[1, 2, 3, 4, 5]
160AEAAAAAKFTGLSKEubiquitination[1, 2, 4]
166AKFTGLSKEELLKVAubiquitination[1, 2, 3, 4]
171LSKEELLKVAGSPGWubiquitination[1, 2, 4]
217CRELPAQKWWHTGALubiquitination[1]
245AGNLAGLKGRLDYLSubiquitination[1, 2]
255LDYLSSLKVKGLVLGubiquitination[1, 2]
257YLSSLKVKGLVLGPIubiquitination[2]
269GPIHKNQKDDVAQTDubiquitination[1, 2]
287IDPNFGSKEDFDSLLubiquitination[1, 2]
298DSLLQSAKKKSIRVIubiquitination[1]
329QVDTVATKVKDALEFubiquitination[1, 2]
354VRDIENLKDASSFLAubiquitination[1, 2, 3]
368AEWQNITKGFSEDRLubiquitination[1, 2, 3]
416GSTGEHTKSLVTQYLubiquitination[1]
615PLELERLKLEPHEGLubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 103 103 Phosphoserine.
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 406 406 Phosphoserine (Probable).
 MOD_RES 408 408 Phosphoserine (Probable).
 MOD_RES 410 410 Phosphoserine (Probable).
 MOD_RES 527 527 Phosphoserine (Probable).
 MOD_RES 531 531 Phosphoserine (Probable).
 CARBOHYD 365 365 N-linked (GlcNAc...).
 CARBOHYD 381 381 N-linked (GlcNAc...).
 CARBOHYD 424 424 N-linked (GlcNAc...).
 CARBOHYD 506 506 N-linked (GlcNAc...).
 DISULFID 210 210 Interchain (with light chain).
 CROSSLNK 147 147 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Alternative splicing; Amino-acid transport; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 630 AA 
Protein Sequence
MELQPPEASI AVVSIPRQLP GSHSEAGVQG LSAGDDSELG SHCVAQTGLE LLASGDPLPS 60
ASQNAEMIET GSDCVTQAGL QLLASSDPPA LASKNAEVTG TMSQDTEVDM KEVELNELEP 120
EKQPMNAASG AAMSLAGAEK NGLVKIKVAE DEAEAAAAAK FTGLSKEELL KVAGSPGWVR 180
TRWALLLLFW LGWLGMLAGA VVIIVRAPRC RELPAQKWWH TGALYRIGDL QAFQGHGAGN 240
LAGLKGRLDY LSSLKVKGLV LGPIHKNQKD DVAQTDLLQI DPNFGSKEDF DSLLQSAKKK 300
SIRVILDLTP NYRGENSWFS TQVDTVATKV KDALEFWLQA GVDGFQVRDI ENLKDASSFL 360
AEWQNITKGF SEDRLLIAGT NSSDLQQILS LLESNKDLLL TSSYLSDSGS TGEHTKSLVT 420
QYLNATGNRW CSWSLSQARL LTSFLPAQLL RLYQLMLFTL PGTPVFSYGD EIGLDAAALP 480
GQPMEAPVML WDESSFPDIP GAVSANMTVK GQSEDPGSLL SLFRRLSDQR SKERSLLHGD 540
FHAFSAGPGL FSYIRHWDQN ERFLVVLNFG DVGLSAGLQA SDLPASASLP AKADLLLSTQ 600
PGREEGSPLE LERLKLEPHE GLLLRFPYAA 630 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005432; F:calcium:sodium antiporter activity; TAS:UniProtKB.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0015175; F:neutral amino acid transmembrane transporter activity; ISS:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
 GO:0016049; P:cell growth; NAS:UniProtKB.
 GO:0060356; P:leucine import; ISS:UniProtKB.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0015827; P:tryptophan transport; ISS:UniProtKB. 
Interpro
 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00128; Alpha-amylase 
SMART
  
PROSITE
  
PRINTS