CPLM-012595

Genbank Nucleotide ID

Protein Synonyms/Alias

Component 3 of promoter of RISC; C3PO

Homo sapiens (Human)

Position	Peptide	Type	References
56	AGFQDIPKRCLKARE	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
187	GFRLLNLKNDSLRKR	acetylation	[8]
187	GFRLLNLKNDSLRKR	ubiquitination	[1, 2, 4, 6, 7]
199	RKRYDGLKYDVKKVE	acetylation	[8]
219	LSIRGFNKETAAACV	ubiquitination	[5, 6]

[1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.

REGION 86 90 DNA/RNA binding.
REGION 177 198 Leucine-zipper (Potential).
MOD_RES 187 187 N6-acetyllysine.
MOD_RES 199 199 N6-acetyllysine.

3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease; Nucleus; Reference proteome; RNA-binding.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; TAS:ProtInc.
GO:0003677; F:DNA binding; TAS:ProtInc.
GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
GO:0003729; F:mRNA binding; IEA:Compara.
GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
GO:0006310; P:DNA recombination; TAS:ProtInc.
GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.

IPR002848; Translin.
IPR016069; Translin_C.
IPR016068; Translin_N.