CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012286
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dynactin subunit 1 
Protein Synonyms/Alias
 150 kDa dynein-associated polypeptide; DAP-150; DP-150; p135; p150-glued 
Gene Name
 DCTN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
230QVRDLEEKLETLRLKacetylation[1]
518ADYQQTIKKYRQLTAubiquitination[2]
558ETFDFKIKFAETKAHubiquitination[2]
567AETKAHAKAIEMELRubiquitination[2]
618LMPRLICKAELIRKQubiquitination[2, 3]
629IRKQAQEKFELSENCubiquitination[2]
962LKKSLKIKGEELSEAubiquitination[2]
1034EAEKAELKQRLNSQSubiquitination[2]
1115QHENSILKGAQMKASubiquitination[2]
1120ILKGAQMKASLASLPubiquitination[2]
1133LPPLHVAKLSHEGPGubiquitination[2]
1151PAGALYRKTSQLLETubiquitination[2]
1179TRTSPAAKSPSAQLMubiquitination[2, 4, 5]
1193MEQVAQLKSLSDTVEubiquitination[2]
1201SLSDTVEKLKDEVLKubiquitination[2]
1203SDTVEKLKDEVLKETubiquitination[2]
1235SSAFLRAKEEQQDDTubiquitination[2, 5]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein- dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. 
Sequence Annotation
 DOMAIN 48 90 CAP-Gly.
 MOD_RES 108 108 Phosphothreonine.
 MOD_RES 541 541 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Amyotrophic lateral sclerosis; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Dynein; Microtubule; Neurodegeneration; Parkinsonism; Phosphoprotein; Polymorphism; Reference proteome; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1278 AA 
Protein Sequence
MAQSKRHVYS RTPSGSRMSA EASARPLRVG SRVEVIGKGH RGTVAYVGAT LFATGKWVGV 60
ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG ADTTSPETPD SSASKVLKRE 120
GTDTTAKTSK LRGLKPKKAP TARKTTTRRP KPTRPASTGV AGASSSLGPS GSASAGELSS 180
SEPSTPAQTP LAAPIIPTPV LTSPGAVPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRAE 240
DKAKLKELEK HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT 300
ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG SDGAASSYQL 360
KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ ELEVVRQQRE RLQEELSQAE 420
STIDELKEQV DAALGAEEMV EMLTDRNLNL EEKVRELRET VGDLEAMNEM NDELQENARE 480
TELELREQLD MAGARVREAQ KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA 540
SVERQQQPPP ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG 600
GDHDCVLVLL LMPRLICKAE LIRKQAQEKF ELSENCSERP GLRGAAGEQL SFAAGLVYSL 660
SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD FLIELLHKDQ LDETVNVEPL 720
TKAIKYYQHL YSIHLAEQPE DCTMQLADHI KFTQSALDCM SVEVGRLRAF LQGGQEATDI 780
ALLLRDLETS CSDIRQFCKK IRRRMPGTDA PGIPAALAFG PQVSDTLLDC RKHLTWVVAV 840
LQEVAAAAAQ LIAPLAENEG LLVAALEELA FKASEQIYGT PSSSPYECLR QSCNILISTM 900
NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET VIKELKKSLK 960
IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLEE TQALLRKKEK EFEETMDALQ 1020
ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG PPPSGIATLV SGIAGEEQQR GAIPGQAPGS 1080
VPGPGLVKDS PLLLQQISAM RLHISQLQHE NSILKGAQMK ASLASLPPLH VAKLSHEGPG 1140
SELPAGALYR KTSQLLETLN QLSTHTHVVD ITRTSPAAKS PSAQLMEQVA QLKSLSDTVE 1200
KLKDEVLKET VSQRPGATVP TDFATFPSSA FLRAKEEQQD DTVYMGKVTF SCAAGFGQRH 1260
RLVLTQEQLH QLHSRLIS 1278 
Gene Ontology
 GO:0031252; C:cell leading edge; IEA:Compara.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0003774; F:motor activity; IEA:UniProtKB-KW.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007067; P:mitosis; NAS:ProtInc.
 GO:0007399; P:nervous system development; NAS:UniProtKB.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 
Interpro
 IPR000938; CAP-Gly_domain.
 IPR022157; Dynactin. 
Pfam
 PF01302; CAP_GLY
 PF12455; Dynactin 
SMART
 SM01052; CAP_GLY 
PROSITE
 PS00845; CAP_GLY_1
 PS50245; CAP_GLY_2 
PRINTS