UniProt Accession

 NMI_HUMAN; Q13287; B5BU69; Q53TI8; Q9BVE5 

Genbank Protein ID

 U32849; AB451305; AB451436; AC009311; CH471058; BC001268 

Genbank Nucleotide ID

 AAC12949.1; BAG70119.1; BAG70250.1; AAY15066.1; EAX11513.1; AAH01268.1 

Protein Name

 N-myc-interactor 

Protein Synonyms/Alias

 Nmi; N-myc and STAT interactor 

Gene Name

 NMI 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
36	LIDEITKKNIQLKKE	ubiquitination	[1]
61	ATKEFQIKEDIPETK	ubiquitination	[1, 2]
68	KEDIPETKMKFLSVE	ubiquitination	[2]
70	DIPETKMKFLSVETP	ubiquitination	[1, 2]
95	CSFQVSSKVPYEIQK	ubiquitination	[1]
102	KVPYEIQKGQALITF	ubiquitination	[1]
111	QALITFEKEEVAQNV	ubiquitination	[1]
123	QNVVSMSKHHVQIKD	ubiquitination	[1]
129	SKHHVQIKDVNLEVT	ubiquitination	[1, 2]
138	VNLEVTAKPVPLNSG	ubiquitination	[1, 2]
158	YVEVSKMKINVTEIP	ubiquitination	[1]
176	REDQMRDKLELSFSK	ubiquitination	[1, 2]
183	KLELSFSKSRNGGGE	ubiquitination	[1, 3]
219	VADKILKKKEYPLYI	ubiquitination	[1, 2, 3]
220	ADKILKKKEYPLYIN	ubiquitination	[1]
244	PYTEIHLKKYQIFSG	ubiquitination	[3, 4, 5, 6]
245	YTEIHLKKYQIFSGT	ubiquitination	[1, 2, 4, 6]
254	QIFSGTSKRTVLLTG	ubiquitination	[1]
284	NIHFQRAKNGGGEVD	ubiquitination	[1, 2, 3]
294	GGEVDVVKCSLGQPH	ubiquitination	[1, 2, 3, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. 

Sequence Annotation

  

Keyword

 Complete proteome; Cytoplasm; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 307 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003712; F:transcription cofactor activity; TAS:ProtInc.
 GO:0006954; P:inflammatory response; TAS:ProtInc.
 GO:0007259; P:JAK-STAT cascade; TAS:UniProtKB.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 

Interpro

 IPR009938; Interferon_induced_35kDa_N.
 IPR009909; Nmi/IFP35. 

Pfam

 PF07334; IFP_35_N
 PF07292; NID 

SMART

  

PROSITE

  

PRINTS