CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004923
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acyl-CoA desaturase 1 
Protein Synonyms/Alias
 Fatty acid desaturase 1; Stearoyl-CoA desaturase 1 
Gene Name
 OLE1 
Gene Synonyms/Alias
 YGL055W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
350TDYRNAIKWYQYDPTacetylation[1]
350TDYRNAIKWYQYDPTubiquitination[2]
375GLAYDLKKFSQNAIEubiquitination[2]
391ALIQQEQKKINKKKAubiquitination[2]
457LIKTALGKDATKAFSubiquitination[2]
461ALGKDATKAFSGGVYubiquitination[2]
487DMRVAVIKESKNSAIubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Utilizes O(2) and electrons from the reduced cytochrome b(5) domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates (Probable). 
Sequence Annotation
 DOMAIN 409 487 Cytochrome b5 heme-binding.
 MOTIF 161 166 Histidine box-1.
 MOTIF 198 202 Histidine box-2.
 MOTIF 335 339 Histidine box-3.
 METAL 444 444 Iron (heme axial ligand) (By similarity).
 METAL 470 470 Iron (heme axial ligand) (By similarity).  
Keyword
 Complete proteome; Electron transport; Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism; Heme; Iron; Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 510 AA 
Protein Sequence
MPTSGTTIEL IDDQFPKDDS ASSGIVDEVD LTEANILATG LNKKAPRIVN GFGSLMGSKE 60
MVSVEFDKKG NEKKSNLDRL LEKDNQEKEE AKTKIHISEQ PWTLNNWHQH LNWLNMVLVC 120
GMPMIGWYFA LSGKVPLHLN VFLFSVFYYA VGGVSITAGY HRLWSHRSYS AHWPLRLFYA 180
IFGCASVEGS AKWWGHSHRI HHRYTDTLRD PYDARRGLWY SHMGWMLLKP NPKYKARADI 240
TDMTDDWTIR FQHRHYILLM LLTAFVIPTL ICGYFFNDYM GGLIYAGFIR VFVIQQATFC 300
INSLAHYIGT QPFDDRRTPR DNWITAIVTF GEGYHNFHHE FPTDYRNAIK WYQYDPTKVI 360
IYLTSLVGLA YDLKKFSQNA IEEALIQQEQ KKINKKKAKI NWGPVLTDLP MWDKQTFLAK 420
SKENKGLVII SGIVHDVSGY ISEHPGGETL IKTALGKDAT KAFSGGVYRH SNAAQNVLAD 480
MRVAVIKESK NSAIRMASKR GEIYETGKFF 510 
Gene Ontology
 GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:SGD.
 GO:0009055; F:electron carrier activity; ISA:SGD.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0004768; F:stearoyl-CoA 9-desaturase activity; IMP:SGD.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0006636; P:unsaturated fatty acid biosynthetic process; IMP:SGD. 
Interpro
 IPR009160; Acyl-CoA_deSatase_haem/ster-bd.
 IPR001199; Cyt_B5-like_heme/steroid-bd.
 IPR018506; Cyt_B5_heme-BS.
 IPR005804; Fatty_acid_desaturase-1.
 IPR001522; Fatty_acid_desaturase-1_C.
 IPR015876; Fatty_acid_desaturase-1_core. 
Pfam
 PF00173; Cyt-b5
 PF00487; FA_desaturase 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2
 PS00476; FATTY_ACID_DESATUR_1 
PRINTS
 PR00075; FACDDSATRASE.