CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036582
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 SUMO-protein ligase NSE2 
Protein Synonyms/Alias
  
Gene Name
 NSMCE2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20RQLNHYVKAVQSTINubiquitination[1]
41PEKIPDLKLLVEKKFubiquitination[2]
47LKLLVEKKFLALQSKubiquitination[3]
54KFLALQSKNSDADFQubiquitination[2, 3, 4]
65ADFQNNEKFVQFKQQacetylation[2, 5]
65ADFQNNEKFVQFKQQubiquitination[1, 2, 3, 4]
70NEKFVQFKQQLKELKubiquitination[1, 3]
74VQFKQQLKELKKQCGubiquitination[3]
123MKKPVKNKVCGHTYEubiquitination[2]
162CSHTDIRKSDLIQDEubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 187 AA 
Protein Sequence
MDKAMVEFAT LDRQLNHYVK AVQSTINHVK EERPEKIPDL KLLVEKKFLA LQSKNSDADF 60
QNNEKFVQFK QQLKELKKQC GLQADREADG TEGVDEDIIV TQSQTNFTCP ITKEEMKKPV 120
KNKVCGHTYE EDAIVRMIES RQKRKKKAYC PQIGCSHTDI RKSDLIQDEA LRRAIENHNK 180
KRHRHSE 187 
Gene Ontology
 GO:0030915; C:Smc5-Smc6 complex; IEA:InterPro.
 GO:0019789; F:SUMO ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000724; P:double-strand break repair via homologous recombination; IEA:InterPro.
 GO:0034184; P:positive regulation of maintenance of mitotic sister chromatid cohesion; IEA:InterPro.
 GO:0045842; P:positive regulation of mitotic metaphase/anaphase transition; IEA:InterPro. 
Interpro
 IPR026846; Nse2(Mms21).
 IPR004181; Znf_MIZ.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
  
SMART
  
PROSITE
 PS51044; ZF_SP_RING 
PRINTS