UniProt Accession

 DPH5_HUMAN; Q9H2P9; A8JZY6; D3DT62; Q9P017; Q9P0I4; Q9Y319 

Genbank Protein ID

 AF132964; AF157319; AF161492; AF248965; AK289351; AC093157; CH471097; CH471097; CH471097; CH471097; BC053857 

Genbank Nucleotide ID

 AAD27739.1; AAF67485.1; AAF29107.1; AAG44563.1; BAF82040.1; EAW72933.1; EAW72937.1; EAW72938.1; EAW72940.1; AAH53857.1 

Protein Name

 Diphthine synthase 

Protein Synonyms/Alias

 Diphthamide biosynthesis methyltransferase 

Gene Name

 DPH5 

Gene Synonyms/Alias

 AD-018; CGI-30; HSPC143; NPD015 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
180	RVGADDQKIAAGTLR	ubiquitination	[1]

Reference

 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis (By similarity). 

Sequence Annotation

 REGION 112 113 S-adenosyl-L-methionine binding (By
 BINDING 9 9 S-adenosyl-L-methionine; via carbonyl
 BINDING 84 84 S-adenosyl-L-methionine; via amide
 BINDING 87 87 S-adenosyl-L-methionine; via carbonyl
 BINDING 163 163 S-adenosyl-L-methionine; via amide
 BINDING 225 225 S-adenosyl-L-methionine; via amide
 BINDING 250 250 S-adenosyl-L-methionine; via carbonyl  

Keyword

 Alternative splicing; Complete proteome; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 285 AA 

Protein Sequence

Gene Ontology

 GO:0004164; F:diphthine synthase activity; NAS:UniProtKB.
 GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; NAS:UniProtKB. 

Interpro

 IPR000878; 4pyrrol_Mease.
 IPR014777; 4pyrrole_Mease_sub1.
 IPR014776; 4pyrrole_Mease_sub2.
 IPR004551; Dphthn_synthase. 

Pfam

 PF00590; TP_methylase 

SMART

  

PROSITE

  

PRINTS