CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018640
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alsin 
Protein Synonyms/Alias
 Amyotrophic lateral sclerosis 2 protein homolog 
Gene Name
 Als2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
527TEVWTWGKGKEGQLGacetylation[1]
660PVLLSCSKLGYISRVubiquitination[2]
843RRLHNYAKVLLKLATubiquitination[2]
987ISSTPQEKTKWLRAIubiquitination[2]
1154GQWVMDKKAGYGVFDubiquitination[2]
1259KITGTYFKPSLYESDubiquitination[2]
1275DKPKAFRKLGNLAVAubiquitination[2]
1368DIKKYLIKACDTPLHubiquitination[2]
1451RRSFCTGKSDSRSESubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 May act as a GTPase regulator (By similarity). Controls survival and growth of spinal motoneurons (By similarity). 
Sequence Annotation
 REPEAT 59 108 RCC1 1.
 REPEAT 109 167 RCC1 2.
 REPEAT 169 218 RCC1 3.
 REPEAT 519 570 RCC1 4.
 REPEAT 572 621 RCC1 5.
 DOMAIN 684 879 DH.
 DOMAIN 895 1001 PH.
 REPEAT 1043 1065 MORN 1.
 REPEAT 1066 1088 MORN 2.
 REPEAT 1094 1116 MORN 3.
 REPEAT 1117 1139 MORN 4.
 REPEAT 1145 1167 MORN 5.
 REPEAT 1169 1191 MORN 6.
 REPEAT 1192 1214 MORN 7.
 REPEAT 1215 1238 MORN 8.
 DOMAIN 1507 1651 VPS9.
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 486 486 Phosphoserine.
 MOD_RES 527 527 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Guanine-nucleotide releasing factor; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1651 AA 
Protein Sequence
MDSKKKSSTE AEGSKERGLV HVWQAGSFSL TPERLPGWGG KTVLQAALGV RHGVLLTEDG 60
EVYSFGTLPW KSESAEICPS SPLLESALVG HHVITVATGS FHSGAVTESG VVYMWGENAA 120
GQCAVANQQY VPEPSPVSIS DSETSPSLAV RILQLACGEE HTLALSLSRE IWAWGTGCQL 180
GLITTTFPVT KPQKVEHLAG RVVLQVACGA FHSLALVQCL PPQDLKPVPE RCNQCSQLLI 240
TMTDKEDHVI ISDSHCCPLG VTLSESQAEK HASPAPSPHP EALDEQGEVF ENTVVEAELN 300
MGSSQTTSGS AISTQQNVVG TAEVSSARTA PSYPDTHAVT AYLQKLSEHS MRENHEPGEK 360
PPQVQPLVEE AVPDLHSPPT TSTSALNSLV VSCASAVGVR VAATYEAGAL SLKKVMNFYS 420
TAPCETAAQS GSASTGPESL KDLREEQVKQ ESLQGKKSSS LMDIREEELE GGSRRLSLPG 480
LLSQVSPRLL RKAARVKTRT VVLTPTYSGE ADALLPSLRT EVWTWGKGKE GQLGHGDVLP 540
RLQPLCVKCL DGKEVIHLEA GGSHSLALTA KSQVYSWGSN TFGQLGHSEF PTTVPRLSKV 600
SSENGVWSVA AGQDYSLFLV DTEDFQPGLY YSGRQDRAEG DTLPENPSGT KTPVLLSCSK 660
LGYISRVTAG KDSYLALVDK NIMGYIASLH ELASTERRFY SKLSEIKSQI LRPLLSLENL 720
GTVTTVQLLQ EVASRFSKLC YLIGQHGASL SSYLQGMKEA SSLVIMKHSS LFLDSYTEYC 780
TSVSNFLVMG GFQLLAKPAI DFLNKNQELL QDLSEVNDEN TQLMEILNML FFLPIRRLHN 840
YAKVLLKLAT CFEVTSPEYQ KLQDSSSCYE SLALHLGKKR KEAEYTLSFW KTFPGKMTDS 900
LRKPERRLLC ESSNRALSLQ HAGRFSVNWF ILFNDALVHA QFSTHHVFPL ATLWAEPLSE 960
EAGSVNGLKI TTPEEQFTLI SSTPQEKTKW LRAISQAVDQ ALRGTSDFPL YGGGSSVQRQ 1020
EPPISRSAKY TFYKDTRLKD ATYDGRWLSG KPHGRGVLKW PDGKMYSGMF RNGLEDGYGE 1080
YRIPNKALNK EDHYVGHWKE GKMCGQGVYS YASGEVFEGC FQDNMRHGHG LLRSGKLTSS 1140
SPSMFIGQWV MDKKAGYGVF DDITRGEKYM GMWQDDVCQG NGVVVTQFGL YYEGNFHLNK 1200
MMGNGVLLSE DDTIYEGEFS DDWTLSGKGT LTMPHGDYIE GYFSGEWGSG IKITGTYFKP 1260
SLYESDKDKP KAFRKLGNLA VAADEKWRAV FEECWRQLGC ESPGQGEVWK AWDNIAVALT 1320
TNRRQHKDSP EILSRSQTQT LESLEYIPQH IGAFSVEKYD DIKKYLIKAC DTPLHPLGRL 1380
VETLVAVYRM TYVGVGANRR LLQEAVKEIK SYLKRIFQLV RFLFPELPEE GSTIPLSAPL 1440
PTGRRSFCTG KSDSRSESPE PGYVVTSSGL LLPVLLPRLY PPLFMLYALD NDREEDIYWE 1500
CVLRLNKQPD IALLGFLGVQ KKFWPATLSI LGESKKVLST TKDACFASAV ECLQQISTTF 1560
TPSDKLKVIQ QTFEEISQSV LASLQEDFLW SMDDLFPVFL YVVLRARIRN LGSEVHLIED 1620
LMDPFLQHGE QGIMFTTLKA CYFQIQREKL N 1651 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005769; C:early endosome; ISS:UniProtKB.
 GO:0030426; C:growth cone; ISS:UniProtKB.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0014069; C:postsynaptic density; IDA:MGI.
 GO:0043234; C:protein complex; ISS:UniProtKB.
 GO:0001726; C:ruffle; IDA:UniProtKB.
 GO:0031982; C:vesicle; ISS:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0043539; F:protein serine/threonine kinase activator activity; ISS:UniProtKB.
 GO:0017137; F:Rab GTPase binding; ISS:UniProtKB.
 GO:0017112; F:Rab guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
 GO:0030676; F:Rac guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
 GO:0007409; P:axonogenesis; IEA:Compara.
 GO:0001662; P:behavioral fear response; IMP:MGI.
 GO:0008219; P:cell death; IEA:Compara.
 GO:0016197; P:endosomal transport; IMP:MGI.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0007626; P:locomotory behavior; IMP:MGI.
 GO:0007528; P:neuromuscular junction development; IMP:MGI.
 GO:0048812; P:neuron projection morphogenesis; ISS:UniProtKB.
 GO:0032855; P:positive regulation of Rac GTPase activity; ISS:UniProtKB.
 GO:0008104; P:protein localization; IMP:MGI.
 GO:0016601; P:Rac protein signal transduction; IEA:Compara.
 GO:0001881; P:receptor recycling; IMP:MGI.
 GO:0051036; P:regulation of endosome size; ISS:UniProtKB.
 GO:0032313; P:regulation of Rab GTPase activity; IDA:MGI.
 GO:0006979; P:response to oxidative stress; IMP:MGI.
 GO:0035249; P:synaptic transmission, glutamatergic; IMP:MGI.
 GO:0016050; P:vesicle organization; IDA:MGI. 
Interpro
 IPR000219; DH-domain.
 IPR003409; MORN.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR009091; RCC1/BLIP-II.
 IPR000408; Reg_chr_condens.
 IPR003123; VPS9. 
Pfam
 PF02493; MORN
 PF00415; RCC1
 PF00621; RhoGEF
 PF02204; VPS9 
SMART
 SM00698; MORN
 SM00233; PH 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS00625; RCC1_1
 PS00626; RCC1_2
 PS50012; RCC1_3
 PS51205; VPS9 
PRINTS
 PR00633; RCCNDNSATION.