CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018113
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) 
Protein Synonyms/Alias
 ATP-dependent dihydroxyacetone kinase; DHA kinase; Glycerone kinase; Triokinase; Triose kinase; FAD-AMP lyase (cyclizing); FAD-AMP lyase (cyclic FMN forming); FMN cyclase 
Gene Name
 Dak 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
5***MSSKKMVNSVEGubiquitination[1]
46RSDLDTLKGRVALLSubiquitination[1]
179MGLEEITKRVSVIAKacetylation[2]
179MGLEEITKRVSVIAKubiquitination[1]
334IDAETTAKAWPHMAKubiquitination[1]
372AAGGVTSKQMALVLDubiquitination[1]
413STHSRAAKAIQGWLKubiquitination[1]
420KAIQGWLKEGPSLTSubiquitination[1]
463TAAAQPLKAKTDLPTubiquitination[1]
465AAQPLKAKTDLPTWSubiquitination[1]
484AGLESMQKYGKAAPGubiquitination[1]
522SLLPVLTKAVKSAEAubiquitination[1]
525PVLTKAVKSAEAAAEacetylation[2]
525PVLTKAVKSAEAAAEubiquitination[1]
535EAAAEATKNMEAGAGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 
Functional Description
 Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate (By similarity). 
Sequence Annotation
 DOMAIN 9 336 DhaK.
 DOMAIN 372 571 DhaL.
 NP_BIND 401 404 ATP (By similarity).
 NP_BIND 446 447 ATP (By similarity).
 NP_BIND 494 495 ATP (By similarity).
 NP_BIND 556 558 ATP (By similarity).
 REGION 56 59 Dihydroxyacetone binding (By similarity).
 ACT_SITE 221 221 Tele-hemiaminal-histidine intermediate
 BINDING 109 109 Dihydroxyacetone (By similarity).
 BINDING 114 114 Dihydroxyacetone (By similarity).
 BINDING 486 486 ATP; via carbonyl oxygen (By similarity).  
Keyword
 ATP-binding; Cobalt; Complete proteome; FAD; Kinase; Lyase; Magnesium; Manganese; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 578 AA 
Protein Sequence
MSSKKMVNSV EGCADDALAG LVASNPDLQL LQGHRVALRS DLDTLKGRVA LLSGGGSGHE 60
PAHAGFIGKG MLTGVIAGSV FASPPVGSIL AAIRAVAQAG TVGTLLIVKN YTGDRLNFGL 120
AMEQAKAEGI SVEMVIVEDD SAFTVLKKAG RRGLCGTVLI HKVAGALAEE GMGLEEITKR 180
VSVIAKTMGT LGVSLSSCSV PGATHTFELA ADEIELGLGI HGEAGVRRIK IAPVDQIVTL 240
MLDHMTNTSN IFHVPVRSGS SVVLIVNNLG GLSFLELGII ADAAIRLLEG RGVKVARALV 300
GTFMSALEMP GVSLTLMLVD EPVLKLIDAE TTAKAWPHMA KVSVTGRKRI RAAPTEPPEA 360
PEATAAGGVT SKQMALVLDR ICTTLIGLEE HLNALDRAAG DGDCGSTHSR AAKAIQGWLK 420
EGPSLTSPAQ VLSRLSVLLL ERMGGSSGAL YGLFLTAAAQ PLKAKTDLPT WSAAMDAGLE 480
SMQKYGKAAP GDRTMLDSLW AAAQEFQAWK SPGASLLPVL TKAVKSAEAA AEATKNMEAG 540
AGRASYISSA QLDQPDPGAV AAAAIFRAIL EVLQTQGA 578 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0034012; F:FAD-AMP lyase (cyclizing) activity; IEA:EC.
 GO:0004371; F:glycerone kinase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050354; F:triokinase activity; IEA:EC.
 GO:0006071; P:glycerol metabolic process; IEA:InterPro. 
Interpro
 IPR004006; Dak1.
 IPR004007; Dak2.
 IPR012734; DhaK_ATP. 
Pfam
 PF02733; Dak1
 PF02734; Dak2 
SMART
  
PROSITE
 PS51481; DHAK
 PS51480; DHAL 
PRINTS