CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010576
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Forkhead box protein K1 
Protein Synonyms/Alias
 Myocyte nuclear factor; MNF 
Gene Name
 FOXK1 
Gene Synonyms/Alias
 MNF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
165FYLRCLGKNGVFVDGubiquitination[1]
388IDPASEAKLVEQAFRubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli (By similarity). 
Sequence Annotation
 DOMAIN 123 175 FHA.
 DNA_BIND 305 400 Fork-head.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 101 101 Phosphoserine.
 MOD_RES 213 213 Phosphoserine.
 MOD_RES 223 223 Phosphoserine.
 MOD_RES 239 239 Phosphoserine.
 MOD_RES 243 243 Phosphoserine.
 MOD_RES 253 253 Phosphoserine.
 MOD_RES 257 257 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 416 416 Phosphoserine.
 MOD_RES 420 420 Phosphoserine (By similarity).
 MOD_RES 428 428 Phosphoserine.
 MOD_RES 436 436 Phosphothreonine.
 MOD_RES 441 441 Phosphoserine.
 MOD_RES 445 445 Phosphoserine.  
Keyword
 Acetylation; Activator; Alternative splicing; Complete proteome; Developmental protein; Differentiation; DNA-binding; Myogenesis; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 733 AA 
Protein Sequence
MAEVGEDSGA RALLALRSAP CSPVLCAAAA AAAFPAAAPP PAPAQPQPPP GPPPPPPPPL 60
PPGAIAGAGS SGGSSGVSGD SAVAGAAPAL VAAAAASVRQ SPGPALARLE GREFEFLMRQ 120
PSVTIGRNSS QGSVDLSMGL SSFISRRHLQ LSFQEPHFYL RCLGKNGVFV DGAFQRRGAP 180
ALQLPKQCTF RFPSTAIKIQ FTSLYHKEEA PASPLRPLYP QISPLKIHIP EPDLRSMVSP 240
VPSPTGTISV PNSCPASPRG AGSSSYRFVQ NVTSDLQLAA EFAAKAASEQ QADTSGGDSP 300
KDESKPPFSY AQLIVQAISS AQDRQLTLSG IYAHITKHYP YYRTADKGWQ NSIRHNLSLN 360
RYFIKVPRSQ EEPGKGSFWR IDPASEAKLV EQAFRKRRQR GVSCFRTPFG PLSSRSAPAS 420
PTHPGLMSPR SGGLQTPECL SREGSPIPHD PEFGSKLASV PEYRYSQSAP GSPVSAQPVI 480
MAVPPRPSSL VAKPVAYMPA SIVTSQQPAG HAIHVVQQAP TVTMVRVVTT SANSANGYIL 540
TSQGAAGGSH DAAGAAVLDL GSEARGLEEK PTIAFATIPA AGGVIQTVAS QMAPGVPGHT 600
VTILQPATPV TLGQHHLPVR AVTQNGKHAV PTNSLAGNAY ALTSPLQLLA TQASSSAPVV 660
VTRVCEVGPK EPAAAVAATA TTTPATATTA SASASSTGEP EVKRSRVEEP SGAVTTPAGV 720
IAAAGPQGPG TGE 733 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005667; C:transcription factor complex; IBA:RefGenome.
 GO:0008301; F:DNA binding, bending; IBA:RefGenome.
 GO:0003690; F:double-stranded DNA binding; IBA:RefGenome.
 GO:0000287; F:magnesium ion binding; IBA:RefGenome.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0008134; F:transcription factor binding; IBA:RefGenome.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0009790; P:embryo development; IBA:RefGenome.
 GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0007389; P:pattern specification process; IBA:RefGenome.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0009888; P:tissue development; IBA:RefGenome. 
Interpro
 IPR000253; FHA_dom.
 IPR008984; SMAD_FHA_domain.
 IPR001766; TF_fork_head.
 IPR018122; TF_fork_head_CS.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00498; FHA
 PF00250; Fork_head 
SMART
 SM00339; FH
 SM00240; FHA 
PROSITE
 PS50006; FHA_DOMAIN
 PS00657; FORK_HEAD_1
 PS00658; FORK_HEAD_2
 PS50039; FORK_HEAD_3 
PRINTS
 PR00053; FORKHEAD.