CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001171
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase ULK1 
Protein Synonyms/Alias
 Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1 
Gene Name
 ULK1 
Gene Synonyms/Alias
 KIAA0722 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
140GIIHRDLKPQNILLSubiquitination[1]
162NPNSIRVKIADFGFAubiquitination[1]
237RLFYEKNKTLVPTIPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. 
Sequence Annotation
 DOMAIN 16 278 Protein kinase.
 NP_BIND 22 30 ATP (By similarity).
 REGION 287 416 Interaction with GABARAP and GABARAPL2.
 ACT_SITE 138 138 Proton acceptor (By similarity).
 BINDING 46 46 ATP (By similarity).
 MOD_RES 317 317 Phosphoserine; by AMPK (By similarity).
 MOD_RES 450 450 Phosphoserine.
 MOD_RES 456 456 Phosphothreonine.
 MOD_RES 467 467 Phosphoserine (By similarity).
 MOD_RES 469 469 Phosphoserine.
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 479 479 Phosphoserine.
 MOD_RES 556 556 Phosphoserine.
 MOD_RES 575 575 Phosphothreonine (By similarity).
 MOD_RES 625 625 Phosphothreonine (By similarity).
 MOD_RES 638 638 Phosphoserine.
 MOD_RES 758 758 Phosphoserine; by MTOR (Probable).  
Keyword
 ATP-binding; Autophagy; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1050 AA 
Protein Sequence
MEPGRGGTET VGKFEFSRKD LIGHGAFAVV FKGRHREKHD LEVAVKCINK KNLAKSQTLL 60
GKEIKILKEL KHENIVALYD FQEMANSVYL VMEYCNGGDL ADYLHAMRTL SEDTIRLFLQ 120
QIAGAMRLLH SKGIIHRDLK PQNILLSNPA GRRANPNSIR VKIADFGFAR YLQSNMMAAT 180
LCGSPMYMAP EVIMSQHYDG KADLWSIGTI VYQCLTGKAP FQASSPQDLR LFYEKNKTLV 240
PTIPRETSAP LRQLLLALLQ RNHKDRMDFD EFFHHPFLDA SPSVRKSPPV PVPSYPSSGS 300
GSSSSSSSTS HLASPPSLGE MQQLQKTLAS PADTAGFLHS SRDSGGSKDS SCDTDDFVMV 360
PAQFPGDLVA EAPSAKPPPD SLMCSGSSLV ASAGLESHGR TPSPSPPCSS SPSPSGRAGP 420
FSSSRCGASV PIPVPTQVQN YQRIERNLQS PTQFQTPRSS AIRRSGSTSP LGFARASPSP 480
PAHAEHGGVL ARKMSLGGGR PYTPSPQVGT IPERPGWSGT PSPQGAEMRG GRSPRPGSSA 540
PEHSPRTSGL GCRLHSAPNL SDLHVVRPKL PKPPTDPLGA VFSPPQASPP QPSHGLQSCR 600
NLRGSPKLPD FLQRNPLPPI LGSPTKAVPS FDFPKTPSSQ NLLALLARQG VVMTPPRNRT 660
LPDLSEVGPF HGQPLGPGLR PGEDPKGPFG RSFSTSRLTD LLLKAAFGTQ APDPGSTESL 720
QEKPMEIAPS AGFGGSLHPG ARAGGTSSPS PVVFTVGSPP SGSTPPQGPR TRMFSAGPTG 780
SASSSARHLV PGPCSEAPAP ELPAPGHGCS FADPITANLE GAVTFEAPDL PEETLMEQEH 840
TEILRGLRFT LLFVQHVLEI AALKGSASEA AGGPEYQLQE SVVADQISLL SREWGFAEQL 900
VLYLKVAELL SSGLQSAIDQ IRAGKLCLSS TVKQVVRRLN ELYKASVVSC QGLSLRLQRF 960
FLDKQRLLDR IHSITAERLI FSHAVQMVQS AALDEMFQHR EGCVPRYHKA LLLLEGLQHM 1020
LSDQADIENV TKCKLCIERR LSALLTGICA 1050 
Gene Ontology
 GO:0005776; C:autophagic vacuole; IDA:UniProtKB.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:Compara.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0034045; C:pre-autophagosomal structure membrane; IDA:UniProtKB.
 GO:0070969; C:ULK1-ATG13-FIP200 complex; IPI:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0000045; P:autophagic vacuole assembly; IEA:Compara.
 GO:0048675; P:axon extension; IEA:Compara.
 GO:0031669; P:cellular response to nutrient levels; ISS:UniProtKB.
 GO:0021707; P:cerebellar granule cell differentiation; IEA:Compara.
 GO:0048671; P:negative regulation of collateral sprouting; IEA:Compara.
 GO:0031175; P:neuron projection development; IMP:UniProtKB.
 GO:0031102; P:neuron projection regeneration; IEA:Compara.
 GO:0016239; P:positive regulation of macroautophagy; IMP:BHF-UCL.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0008104; P:protein localization; IMP:UniProtKB.
 GO:0021933; P:radial glia guided migration of cerebellar granule cell; IEA:Compara.
 GO:0007265; P:Ras protein signal transduction; IEA:Compara.
 GO:0031623; P:receptor internalization; IEA:Compara.
 GO:0051386; P:regulation of neurotrophin TRK receptor signaling pathway; IEA:Compara.
 GO:0042594; P:response to starvation; ISS:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR016237; Ser/Thr_kin_STPK_Ulk-1/2.
 IPR008271; Ser/Thr_kinase_AS.
 IPR022708; Ser/Thr_kinase_C. 
Pfam
 PF12063; DUF3543
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS