CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018894
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polyribonucleotide 5'-hydroxyl-kinase Clp1 
Protein Synonyms/Alias
 Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Pre-mRNA cleavage complex II protein Clp1 
Gene Name
 CLP1 
Gene Synonyms/Alias
 HEAB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
281VRTVLLPKSGGVVERubiquitination[1]
325NVKFSDVKIYKVGAPubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA- induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. 
Sequence Annotation
 NP_BIND 124 129 ATP (By similarity).
 BINDING 22 22 ATP (By similarity).
 BINDING 62 62 ATP; via carbonyl oxygen (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Magnesium; Manganese; mRNA processing; Nickel; Nucleotide-binding; Nucleus; Reference proteome; Transferase; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 425 AA 
Protein Sequence
MGEEANDDKK PTTKFELERE TELRFEVEAS QSVQLELLTG MAEIFGTELT RNKKFTFDAG 60
AKVAVFTWHG CSVQLSGRTE VAYVSKDTPM LLYLNTHTAL EQMRRQAEKE EERGPRVMVV 120
GPTDVGKSTV CRLLLNYAVR LGRRPTYVEL DVGQGSVSIP GTMGALYIER PADVEEGFSI 180
QAPLVYHFGS TTPGTNIKLY NKITSRLADV FNQRCEVNRR ASVSGCVINT CGWVKGSGYQ 240
ALVHAASAFE VDVVVVLDQE RLYNELKRDL PHFVRTVLLP KSGGVVERSK DFRRECRDER 300
IREYFYGFRG CFYPHAFNVK FSDVKIYKVG APTIPDSCLP LGMSQEDNQL KLVPVTPGRD 360
MVHHLLSVST AEGTEENLSE TSVAGFIVVT SVDLEHQVFT VLSPAPRPLP KNFLLIMDIR 420
FMDLK 425 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000214; C:tRNA-intron endonuclease complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; TAS:UniProtKB.
 GO:0046404; F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; IEA:EC.
 GO:0051736; F:ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; IDA:UniProtKB.
 GO:0051733; F:polydeoxyribonucleotide kinase activity; IDA:UniProtKB.
 GO:0031124; P:mRNA 3'-end processing; TAS:UniProtKB.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0035087; P:siRNA loading onto RISC involved in RNA interference; IDA:UniProtKB.
 GO:0030423; P:targeting of mRNA for destruction involved in RNA interference; IMP:UniProtKB.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome.
 GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IDA:UniProtKB. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR010655; Pre-mRNA_cleavage_cplxII_Clp1. 
Pfam
 PF06807; Clp1 
SMART
  
PROSITE
  
PRINTS