CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023174
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mediator of RNA polymerase II transcription subunit 23 
Protein Synonyms/Alias
 Activator-recruited cofactor 130 kDa component; ARC130; Cofactor required for Sp1 transcriptional activation subunit 3; CRSP complex subunit 3; Mediator complex subunit 23; Protein sur-2 homolog; hSur-2; Transcriptional coactivator CRSP130; Vitamin D3 receptor-interacting protein complex 130 kDa component; DRIP130 
Gene Name
 MED23 
Gene Synonyms/Alias
 ARC130; CRSP3; DRIP130; KIAA1216; SUR2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
242GAICNSWKLDPATLRubiquitination[1]
253ATLRFPLKGLLPYDKubiquitination[1, 2, 3]
260KGLLPYDKDLFEPQTubiquitination[2, 3]
291CNMLGLNKQHKQRCPubiquitination[1]
294LGLNKQHKQRCPVLEubiquitination[1]
465LQQSLRNKSLQMNDYubiquitination[1, 3]
554VIKLAHAKSSVALAPubiquitination[3]
673TRFLSDPKTVLSAESubiquitination[1, 3]
758ESRFNLKKNVEEEYRubiquitination[1]
905NRVSDFVKENSPEHWubiquitination[1]
1050CLSDTYLKCAMNAREubiquitination[1]
1082LVDTMAGKSPGPFPNubiquitination[1]
1132ALLNVVLKSQPLVPRubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Required for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling. 
Sequence Annotation
  
Keyword
 Activator; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Mental retardation; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1368 AA 
Protein Sequence
METQLQSIFE EVVKTEVIEE AFPGMFMDTP EDEKTKLISC LGAFRQFWGG LSQESHEQCI 60
QWIVKFIHGQ HSPKRISFLY DCLAMAVETG LLPPRLVCES LINSDTLEWE RTQLWALTFK 120
LVRKIIGGVD YKGVRDLLKV ILEKILTIPN TVSSAVVQQL LAAREVIAYI LERNACLLPA 180
YFAVTEIRKL YPEGKLPHWL LGNLVSDFVD TFRPTARINS ICGRCSLLPV VNNSGAICNS 240
WKLDPATLRF PLKGLLPYDK DLFEPQTALL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL 300
EDQLVDLVVY AMERSETEEK FDDGGTSQLL WQHLSSQLIF FVLFQFASFP HMVLSLHQKL 360
AGRGLIKGRD HLMWVLLQFI SGSIQKNALA DFLPVMKLFD LLYPEKEYIP VPDINKPQST 420
HAFAMTCIWI HLNRKAQNDN SKLQIPIPHS LRLHHEFLQQ SLRNKSLQMN DYKIALLCNA 480
YSTNSECFTL PMGALVETIY GNGIMRIPLP GTNCMASGSI TPLPMNLLDS LTVHAKMSLI 540
HSIATRVIKL AHAKSSVALA PALVETYSRL LVYMEIESLG IKGFISQLLP TVFKSHAWGI 600
LHTLLEMFSY RMHHIQPHYR VQLLSHLHTL AAVAQTNQNQ LHLCVESTAL RLITALGSSE 660
VQPQFTRFLS DPKTVLSAES EELNRALILT LARATHVTDF FTGSDSIQGT WCKDILQTIM 720
SFTPHNWASH TLSCFPGPLQ AFFKQNNVPQ ESRFNLKKNV EEEYRKWKSM SNENDIITHF 780
SMQGSPPLFL CLLWKMLLET DHINQIGYRV LERIGARALV AHVRTFADFL VYEFSTSAGG 840
QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV 900
SDFVKENSPE HWLQNDWHTK HMNYHKKYPE KLYFEGLAEQ VDPPVQIQSP YLPIYFGNVC 960
LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR PVTYLYNTLH YYEMHLRDRA 1020
FLKRKLVHAI IGSLKDNRPQ GWCLSDTYLK CAMNAREENP WVPDDTYYCR LIGRLVDTMA 1080
GKSPGPFPNC DWRFNEFPNP AAHALHVTCV ELMALAVSGK EVGNALLNVV LKSQPLVPRE 1140
NITAWMNAIG LIITALPEPY WIVLHDRIVS VISSPSLTSE TEWVGYPFRL FDFTACHQSY 1200
SEMSCSYTLA LAHAVWHHSS IGQLSLIPKF LTEVLLPIVK TEFQLLYVYH LVGPFLQRFQ 1260
QERTRCMIEI GVAFYDMLLN VDQCSTHLNY MDPICDFLYH MKYMFTGDSV KEQVEKIICN 1320
LKPALKLRLR FITHISKMEP AAVPPQAMNS GSPAPQSNQV PVSLPVTQ 1368 
Gene Ontology
 GO:0016592; C:mediator complex; IEA:Compara.
 GO:0005667; C:transcription factor complex; IDA:MGI.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 
Interpro
 IPR021629; Mediator_Med23. 
Pfam
 PF11573; Med23 
SMART
  
PROSITE
  
PRINTS