CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023364
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative DNA helicase Ino80 
Protein Synonyms/Alias
 dINO80 
Gene Name
 Ino80 
Gene Synonyms/Alias
 CG31212 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
497RAFDVFAKKKEKEEEacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 DNA helicase and probable main scaffold component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair (By similarity). 
Sequence Annotation
 DOMAIN 313 438 DBINO.
 DOMAIN 547 718 Helicase ATP-binding.
 DOMAIN 1160 1315 Helicase C-terminal.
 NP_BIND 560 567 ATP (By similarity).
 MOD_RES 47 47 Phosphoserine.
 MOD_RES 48 48 Phosphoserine.
 MOD_RES 52 52 Phosphothreonine.
 MOD_RES 67 67 Phosphoserine.
 MOD_RES 70 70 Phosphoserine.
 MOD_RES 227 227 Phosphoserine.
 MOD_RES 230 230 Phosphoserine.  
Keyword
 ATP-binding; Chromatin regulator; Coiled coil; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1638 AA 
Protein Sequence
MEAKPVPAPQ PRPRAMAEPL HIQRLEAALN MRPFMNMAKR SLRKPLSSDE ETDDEHVVKR 60
EHDVQDSDDS STVGVVRMKQ SSKRKSRLLA SKEERQSVKA QLYNFNDLTS DREWLYDLLL 120
SDTESDDPTI TEDEYVQQLL REHVREQRQR KNYYKKAANA QYAYYGSGLL SNHDIFAERQ 180
LATAGVRKRR RRTKQEILMA RLAEAQAGPK PPKQRRRGRK KRDNMGSPES GEVPPSELGK 240
YTFGDTLPNN EDDDEDGGEV DYKRELASLA LDYPEEEEIE EEVDVEGGTE GQVTKVRRKR 300
KNPAALAARR RRIWQIMSKK ESGRLQRIKS NNHKEMLANC KRVAGMCAKV VRQRAINSQR 360
IMKETVWRAK RLTREMLAYW KRYERVERDQ RRKQEREAEE QRKQDVELIE VKRQQRKLNF 420
LITQTELYAH FMSKKLGQGS EEDQLRILSQ LDEETNARLA AQDDYDAGEM KLLAQENAEA 480
AMQRDLDKTR AFDVFAKKKE KEEEEQAQES VEDIKPEPRP EMKDLPQPKM FKGTLKGYQI 540
KGMTWLANIY DQGISGILAD EMGLGKTVQS IAFLCHIAEH YGVWGPFLVI SPASTLHNWQ 600
QEMSRFVPDF KVVPYWGSPA ERKILRQFWD QKHLHTRDAS FHVVITSYQL VVSDYKYFNR 660
IKWQYMVLDE AQAIKSAASQ RWKLLLGFSC RNRLLLSGTP IQNSMAELWA LLHFIMPTLF 720
DSHDEFNEWF SKDIESHAEN KTGIDEKQIS RLHMILKPFM LRRIKKDVEN ELSDKIEIMV 780
YCPLTIRQKL LYRALKQKIR IEDLLHLTSG STTTSSSSSA SNLMNLVMQF RKVCNHPELF 840
ERRDARSPFF MRCAEYTIPR LIHEEGLIHR MLPSRKHLLY NRFNIFKSEY IQRSLFEDVN 900
VNSCFGFTRL CDLSVGDMVE VTLNGLIDFL LHYRRVLEKY PLLAYRRFWW KKQPDSRYQL 960
LEPMLENKLA LDYMPPNSVL KNFIFTAMTA NESSVYAFGD YFTYNMQETI EHRVIRSKIL 1020
KKKTSLIEEM EDVSKQKLEI ESVEVQTKSN AKSDVKVTTL LLLPEFPHRP RKPRKYVCEP 1080
LSMPRILYDL GQKVQAVHRY LYCDSRSAAW SQIRHNQCEN SQGRELVSSG LALCKPHGGW 1140
SSIVVPDKET LITDAGKLFV LDNLLTRLKA NGHRVLIYSQ MTKMIDLLEE YMWHRKHRYM 1200
RLDGSSKISA RRDMVADFQT RADIFVFLLS TRAGGLGINL TAADTVIFYD SDWNPTVDQQ 1260
AMDRAHRLGQ TKQVTVYRLI CKGTIEERIL QRAREKSEIQ RMVISGGNFK PDTLKPKEVV 1320
SLLLDDEEIE MKYRQEAKLQ SSSPIPAATQ SERKRRHPQK DVNMGGTTIA ATSATQNPDD 1380
DVPSCSSAAK RIKLETEEDF IDVGITSSAS SVGTDSNHPT LSQETYVPGA TCVQQTEIDS 1440
ENEALVVDGD SPTMLGQNES MNFLDDLSGI SPMRRRHHPR GTRRGRPRGS TRRGGGHGSI 1500
PRVLTPTQAA TPAVPATASQ AAAAGTGAAA GTSSPLPQQE VSGGGDNAGV PLHEEEYRTS 1560
PSGQSPGVSC KRGPGRPRSK TATPISRGTR GAPRARRPMG PLLVPLGRSP DDTPPSSSPA 1620
TSRAPSPLSG SHGAVGPE 1638 
Gene Ontology
 GO:0031011; C:Ino80 complex; IDA:FlyBase.
 GO:0005700; C:polytene chromosome; IDA:FlyBase.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; ISS:UniProtKB.
 GO:0003678; F:DNA helicase activity; ISS:UniProtKB.
 GO:0000975; F:regulatory region DNA binding; IDA:FlyBase.
 GO:0006338; P:chromatin remodeling; ISS:UniProtKB.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0010468; P:regulation of gene expression; IMP:FlyBase.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR020838; DBINO.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF13892; DBINO
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51413; DBINO
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS