CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003988
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polyadenylate-binding protein 1 
Protein Synonyms/Alias
 PABP-1; Poly(A)-binding protein 1 
Gene Name
 PABPC1 
Gene Synonyms/Alias
 PAB1; PABP1; PABPC2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78TMNFDVIKGKPVRIMubiquitination[1]
104GVGNIFIKNLDKSIDubiquitination[1]
188AELGARAKEFTNVYIubiquitination[1]
213RLKDLFGKFGPALSVubiquitination[1]
284KRKFEQMKQDRITRYubiquitination[1]
299QGVNLYVKNLDDGIDubiquitination[1]
312IDDERLRKEFSPFGTubiquitination[1]
361NGRIVATKPLYVALAubiquitination[1]
536QAKEAAQKAVNSATGubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre- mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. 
Sequence Annotation
 DOMAIN 11 89 RRM 1.
 DOMAIN 99 175 RRM 2.
 DOMAIN 191 268 RRM 3.
 DOMAIN 294 370 RRM 4.
 DOMAIN 542 619 PABC.
 REGION 166 289 CSDE1-binding.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 116 116 Phosphotyrosine (By similarity).
 MOD_RES 299 299 N6-methyllysine.
 MOD_RES 315 315 Phosphoserine.
 MOD_RES 455 455 Omega-N-methylated arginine; by CARM1;
 MOD_RES 460 460 Omega-N-methylated arginine; by CARM1;
 MOD_RES 493 493 Dimethylated arginine; alternate.
 MOD_RES 493 493 Omega-N-methylarginine; alternate.
 MOD_RES 512 512 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Methylation; mRNA processing; mRNA splicing; Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 636 AA 
Protein Sequence
MNPSAPSYPM ASLYVGDLHP DVTEAMLYEK FSPAGPILSI RVCRDMITRR SLGYAYVNFQ 60
QPADAERALD TMNFDVIKGK PVRIMWSQRD PSLRKSGVGN IFIKNLDKSI DNKALYDTFS 120
AFGNILSCKV VCDENGSKGY GFVHFETQEA AERAIEKMNG MLLNDRKVFV GRFKSRKERE 180
AELGARAKEF TNVYIKNFGE DMDDERLKDL FGKFGPALSV KVMTDESGKS KGFGFVSFER 240
HEDAQKAVDE MNGKELNGKQ IYVGRAQKKV ERQTELKRKF EQMKQDRITR YQGVNLYVKN 300
LDDGIDDERL RKEFSPFGTI TSAKVMMEGG RSKGFGFVCF SSPEEATKAV TEMNGRIVAT 360
KPLYVALAQR KEERQAHLTN QYMQRMASVR AVPNPVINPY QPAPPSGYFM AAIPQTQNRA 420
AYYPPSQIAQ LRPSPRWTAQ GARPHPAVHV QGQEPLTASM LASAPPQEQK QMLGERLFPL 480
IQAMHPTLAG KITGMLLEID NSELLHMLES PESLRSKVDE AVAVLQAHQA KEAAQKAVNS 540
ATGVPTV 547 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0008143; F:poly(A) RNA binding; TAS:UniProtKB.
 GO:0008494; F:translation activator activity; TAS:UniProtKB.
 GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
 GO:0006378; P:mRNA polyadenylation; TAS:UniProtKB.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB.
 GO:0048255; P:mRNA stabilization; TAS:UniProtKB.
 GO:2000623; P:negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; ISS:UniProtKB.
 GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISS:UniProtKB.
 GO:0006413; P:translational initiation; TAS:Reactome. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR006515; PABP_1234.
 IPR002004; PABP_HYD.
 IPR000504; RRM_dom. 
Pfam
 PF00658; PABP
 PF00076; RRM_1 
SMART
 SM00517; PolyA
 SM00360; RRM 
PROSITE
 PS51309; PABC
 PS50102; RRM 
PRINTS